硫化氢对代谢综合征小鼠血糖和血胰岛素水平的调节

目的 观察硫化氢（H2S）对代谢综合征小鼠血糖和血胰岛素水平的影响及可能机制.方法 8～10周的雄性C57BL/6J小鼠10只作为正常对照组（CON组）,同周龄雄性KK/Upj-Ay/J小鼠随机分为MS＋NaHS组和MS组,每组各6只,MS＋NaHS组每日腹腔注射0.25 mL H2S供体NaHS（78 μmol/kg）,CON组和MS组每日腹腔注射等量生理盐水,均连续干预4周.采用ONE TOUCH Ultra血糖仪测定血糖值,放射免疫分析法测定胰岛素值,荧光显微镜观察小鼠C2C12骨骼肌细胞株对2-脱氧荧光葡萄糖（2-NBDG）的摄取能力.结果 KK/Upj-Ay/J小鼠的腹围、Lee指数和空腹血糖、胰岛素以及三酰甘油、总胆固醇、低密度脂蛋白胆固醇均比CON组显著升高（P＜ 0.01）;MS＋NaHS组小鼠空腹血糖和胰岛素比MS组分别降低24.2％和46.98％（P＜ 0.05）;NaHS干预后,骨骼肌摄取葡萄糖增加（P＜0.05）;在高糖（16.7 mmol/L）刺激下,不同浓度NaHS干预后,胰岛素分泌水平下降（P＜0.05）.结论 NaHS可以促进骨骼肌细胞对葡萄糖的摄取,同时可以直接抑制高糖刺激下的胰岛素分泌.

Effect of hydrogen sulfide on fasting blood glucose and serum insulin in MS mice

Objective To explore the effect of hydrogen sulfide on fasting blood glucose and serum insulin in MS mice and its possible mechanism.Methods C57BL/6J mice （8-10 weeks） were selected in the control group （CON group,n=10）.KK/UpjAy/J mice （8-10 weeks） were randomly divided into the MS ＋ NaHS group （n=6） and MS group （n=6）.MS ＋ NaHS group was given an injection of 0.25 mL NaHS （78 μmol/kg） daily for 4 weeks.The CON group and MS group were given an injection of 0.25 mL saline daily for 4 weeks.The fasting blood glucose level was determined by ONE TOUCH Ultra and the serum insulin level was determined by radioimmunoassay.2-NBDG uptake of C2C12 was measured by fluorescence microscope.Results The abdomen circumference,Lee＇s index,FBG,insulin level,TC,TG,LDL-C of KK/Upj-Ay/J mice were all higher than these in CON group （P 〈 0.01）.Compared to MS group,the FBG and insulin level in MS＋NaHS group reduced to 24.2%and 46.98% （P 〈 0.05）.2-NBDG uptaked of C2C12 increased in MS＋NaHS group （P 〈 0.05）.Insulin secretion levels dropped on the effect of different levels of NaHS under the stimulate of 16.7 mmol/L glucose （P 〈 0.05）.Conclusion NaHS can advance the 2-NBDG uptake of C2C12,but restrain the insulin secretion levels under the stimulate of high glucose.