氧化低密度脂蛋白诱导小鼠心脏内皮细胞炎性反应中Toll样受体2的表达

目的 研究氧化低密度脂蛋白（ox-LDL）诱导小鼠心脏内皮细胞炎性反应中Toll样受体（TLR）2的表达.方法 分离小鼠心脏内皮细胞,对照组给予DMEM培养,干预组分别给予不同浓度的ox-LDL（20,40 μg/mL）作用细胞24 h,采用Western blot法检测细胞间黏附分子-1（ICAM-1）、TLR2的表达,利用ELISA方法检测炎症因子白细胞介素（IL）-6、IL-8的变化.结果 与对照组相比,ox-LDL干预组TLR2蛋白表达减少（P＜ 0.01）,ICAM-1蛋白表达增多（P＜ 0.01）,IL-6和IL-8表达明显增加（P＜0.05）;与低浓度组比较,高低浓度组ICAM-1、IL-6和IL-8表达差异无统计学意义（P＞0.05）.结论 ox-LDL可以诱导小鼠心脏内皮细胞的炎性反应,同时可能抑制了TLR2受体的表达.

The expression of TLR2 receptor in cardiac endothelial cells isolated from mice with inflammatory response induced by ox-LDL

Objective To research the expression of TLR2 receptor in cardiac endothelial cells isolated from mice with inflammatory response induced by ox-LDL.Methods Cardiac endothelial cells were isolated from mice,ox-LDL （20,40 μg/mL）was added into the medium for 24 h retrospectively in the treatment group,while only DMEM was added in the control group.Chemokine ICAM-1 and TLR2 receptor was detected by Western blot,cytokine IL-6 and IL-8 were determined by ELISA.Results Compared with the control group,ICAM-1,IL-6 and IL-8 increased in the ox-LDL treatment group （P 〈 0.05 or P 〈0.01）,while TLR2 receptor decreased in the ox-LDL treatment group （P 〈 0.01）.There were no differences between low and high concentration group in the expression of ICAM-1,IL-6 and IL-8 （P 〉 0.05）.Conclusion ox-LDL can induce inflammatory response in cardiac endothelial cells isolated from mice,while may inhibit the expression of TLR2 in cells.