| 糖基在小鼠Lewis肺癌细胞与层粘连蛋白识别及结合中的作用 |
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| 引用本文： | 张青云,周柔丽.糖基在小鼠Lewis肺癌细胞与层粘连蛋白识别及结合中的作用[J].北京大学学报(医学版),1990(6). |
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| 作者姓名： | 张青云 周柔丽 |
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| 作者单位： | 北京医科大学细胞生物学教研室
(张青云)，北京医科大学细胞生物学教研室(周柔丽) |
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| 摘 要： | 培养的小鼠Lewis肺癌细胞可粘着于固化在玻璃表面的层粘连蛋白(Laminin,LN)基质上。此过程可分别被抗LN兔血清和抗LN受体(LN-R)兔血清阻断,说明二者的结合是LN与细胞表面LN-R的作用结果。几种外源性单糖如唾液酸,α-甲基甘露糖等可抑制Lewis肺癌细胞与LN相结合,且有剂量依赖性。一种混合糖肽可分别与Lewis肺癌细胞和LN结合,而阻断二者的结合。
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| 关 键 词： | Lewis肺癌 层粘连蛋白 层粘连蛋白受体 糖肽 |
THE ROLES OF SACCHARIDES IN THE RECOGNITION AND BINDING OF MURINE LEWIS LUNG CARCINOMA CELLS WITH LAMININ |
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| Zhang Qingyun,Zhou Rouli.THE ROLES OF SACCHARIDES IN THE RECOGNITION AND BINDING OF MURINE LEWIS LUNG CARCINOMA CELLS WITH LAMININ[J].Journal of Peking University:Health Sciences,1990(6). |
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| Authors: | Zhang Qingyun Zhou Rouli |
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| Institution: | Zhang Qingyun;Zhou Rouli Department of Cell Biology |
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| Abstract: | The cultured murine Lewis lung carcinoma cells may bind to wlaminin(LN)immobilized. The bindingwas inhibited by anti-LN and anti-LN-R serum from rabbit, respectively, which indicates that the bindingis the interaction of LN with its receptor on the cell surface. Serveral exogenous monosaccharides sucha8 N-acetyl-neuraminic acid, alpha-methyl-D-mannopyranoside etc. inhibited the binding of Lewis lungcarcinoma cells with LN with dose-depedent. the glycopeptides isolated from Lewis lung carcinoma maybind to murine Lewis lung carcinoma cells and LN, respectively and remarkably inhibited the interact-ion of the cells with LN. |
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| Keywords: | Lewis lung carcinoma Laminin Laminin receptor Glycopeptide |
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