胰腺癌组织中p57kip2、cyclinE和PCNA表达与临床病理因素的关系

背景与目的:细胞周期调控异常、细胞过度增殖与肿瘤发生密切相关 ,p57kip2蛋白作为细胞周期负性调控因子与胰腺癌的关系尚很少报道.本研究的目的是探讨 p57kip2、 cyclin E蛋白和增殖细胞核抗原( proliferating cell nuclear antigen,PCNA)在胰腺癌发生、发展中的作用.方法:应用免疫组织化学技术( SP法)对 32例胰腺癌组织及癌旁组织中 p57kip2、 cyclin E蛋白和 PCNA表达进行检测.结果: p57kip2蛋白在胰腺癌组织中的阳性表达率为 46.9% ,显著低于癌旁胰腺组织 (75.0% )(χ 2=5.317,P< 0.05),并与胰腺癌组织分化程度有关 (P< 0.05),而与淋巴结转移情况无关 (P >0.05); cyclin E蛋白阳性表达率在胰腺癌组织中为 68 8%,显著高于癌旁胰腺组织 (43.8% )(χ 2=4 063,P< 0.05) ,并与胰腺癌组织分化程度和淋巴结转移情况相关 (P< 0.05);PCNA阳性表达率在胰腺癌组织中为 71.9%,显著高于癌旁胰腺组织 (43.8% )(χ 2=5.189,P< 0.05),并与胰腺癌组织分化程度和淋巴结转移情况有关 (P< 0.05). p57kip2阳性胰腺癌组织中 cyclin E蛋白阳性表达率 ( 60.0%)低于 p57kip2阴性胰腺癌组织中 cyclin E蛋白阳性表达率( 76.5%) ,但两者无相关 (r=- 0 11211,P >0.05).结论: p57kip2蛋白低表达和 cyclin E、 PCNA蛋白过度表达与胰腺癌的发生、发展有关.

Relationship between expression of p57(kip2), cyclin E protein,PCNA, and clinicopathological factors in human pancreatic cancer

BACKGROUND & OBJECTIVE: The abnormality of mammalian cell cycle regulation is an important cause of cell over-proliferation and oncogenesis. There were few reports about the relationship between p57(kip2) protein as negative factor of cell cycle regulation and pancreatic cancer. This article aims to investigate the effects of p57(kip2), cyclin E and proliferating cell nuclear antigen (PCNA) protein on the occurrence and progression of pancreatic cancer. METHODS: Expression of p57(kip2), cyclin E, and PCNA in tumor tissue and adjacent tissue from 32 patients with pancreatic cancer were detected using SP immunohistochemical technique. RESULTS: The positive-expression rate of p57(kip2) protein in tumor tissue of pancreatic cancer was 46.9%, which was lower than that in adjacent pancreatic tissue (75.0%) (Chi(2)=5.317, P< 0.05); p57(kip2) protein positive-expression was remarkably correlated with tumor cell differentiation (P< 0.05), but was not correlated with lymph node metastasis (P >0.05). The positive-expression rate of cyclin E in tumor tissues was 68.8%, which was higher than that in adjacent pancreatic tissue (43.8%) (Chi(2)=4.063,P< 0.05); Cyclin E positive-expression was remarkably correlated with tumor cell differentiation and lymph node metastasis (P< 0.05). The positive-expression rate of PCNA protein in tumor tissues was 71.9%, which was higher than that in adjacent pancreatic tissue (43.8%) (Chi(2)=5.189,P< 0.05); PCNA positive- expression was remarkably correlated with tumor cell differentiation and lymph node metastasis(P< 0.05).CONCLUSION: The decreased expression of p57(kip2) protein and over-expression of cyclin E and PCNA proteins may significantly related to genesis and progress of pancreatic cancer.