| 大鼠内淋巴囊的增殖细胞 |
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| 引用本文： | 黄孝文,肖红俊,汪吉宝,黄翔.大鼠内淋巴囊的增殖细胞[J].临床耳鼻咽喉头颈外科杂志,2000,14(5):218-219. |
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| 作者姓名： | 黄孝文 肖红俊 汪吉宝 黄翔 |
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| 作者单位： | 同济医科大学附属协和医院耳鼻咽喉科,武汉,430022 |
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| 摘 要： | 目的：了解大鼠内淋巴囊的细胞增殖状态。方法：选用１０只健康成年Ｓ－Ｄ大鼠，腹腔注射５－溴－２‘－脱氧尿嘧啶核苷后，取颞骨作组织学处理，用抗ＢｒｄＵｒｄ单克隆抗体，通过免疫组织化学技术，观察内淋巴囊Ｓ期细胞的定位与分析。结论：大鼠内淋巴囊具有细胞增殖功能，可能是内耳具有细胞更新功能的重要部位。
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| 关 键 词： | 内淋巴囊 细胞增殖 BrdUrd 免疫组织化学 内耳 |
The proliferating cells in the rat endolymphatic sac |
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| HUANG Xiao-wen,XIAO Hong-jun,WANG Ji-bao,HUANG Xiang.The proliferating cells in the rat endolymphatic sac[J].Journal of Clinical Otorhinolaryngology,2000,14(5):218-219. |
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| Authors: | HUANG Xiao-wen XIAO Hong-jun WANG Ji-bao HUANG Xiang |
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| Institution: | Department of Otolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022. |
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| Abstract: | OBJECTIVE: To study the cellular proliferating activity of the endolymphatic sac. METHOD: 10 healthy adult S-D rats were used for the experiment. After administration of 5-bromo-2'-deoxyuridine (BrdUrd) intraperitoneally, the rats were sacrificed and the temporal bones were removed, then the decalcified samples examined immunohistochemically by using monoclonal antibodies against BrdUrd. RESULT: Scattered BrdUrd+ cells were detected within the endolymrhatic sac (ES) whereas labelled cells were not fonnd in the cochlea. CONCLUSION: Possessing a low rat of mitotic activity, the ES may be an important the sole site for the cell-renewing in the inner ear. |
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| Keywords: | Endolymphatic sac Cellular proliferation Bromodeoxyuridine Immunohistochemitry |
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