丹栀逍遥散对人乳腺癌MCF-7细胞株裸鼠移植瘤的影响

目的:研究丹栀逍遥散对MCF-7乳腺癌细胞株裸鼠移植瘤的抑制作用及其机制。方法:体外培养MCF-7人乳腺癌细胞,将MCF-7细胞悬液接种于裸鼠腋下,建立裸鼠皮下移植瘤模型。雌性裸鼠分为4组,即模型组,丹栀逍遥散高、中、低剂量(17.98,8.99,4.50 g·kg~(-1))组,每组8只,每日ig给予丹栀逍遥散连续15 d,模型组ig等容积的生理盐水。比较给药各组肿瘤质量抑制率,采用苏木素和伊红(HE)染色法观察给药各组对肿瘤组织形态学影响,采用Tunel染色法观察给药各组对肿瘤细胞凋亡的影响。结果:与模型组比较,丹栀逍遥散高、中剂量组对肿瘤组织质量有显著抑制作用(P0.05,P0.01),可改善肿瘤组织形态学,增加肿瘤组织被Tunel染色的细胞数目,增加肿瘤细胞凋亡率(P0.01)。结论:丹栀逍遥散能够抑制MCF-7荷瘤裸鼠肿瘤组织生长,其作用机制可能与其促进肿瘤细胞凋亡有关。

Effect of Danzhi Xiaoyao San on Human Breast Cancer Cell Line MCF-7 in Nude Mice

Objective:To investigate the inhibitory effect and mechanism of Danzhi Xiaoyao San(DZXYS) on human breast cancer cell line MCF-7 in nude mice. Method:MCF-7 cells were inoculated in nude mice armpit to establish the subcutaneous tumor model. Female mice were divided to four groups randomly:model group, high-dose DZXYS group (17.98 g·kg^-1), middle-dose DZXYS group (8.99 g·kg^-1), and low-dose DZXYS group (4.50 g·kg^-1). Mice of high, middle and low-dose DZXYS groups were orally administrated with DZXYS and model groups with equivalent saline. The treatment course was 15 days. The tumor inhibition rate of tumor mass was observed. Tumor tissue morphology was observed by hematoxylin and eosin(HE) staining. Apoptosis of tumor tissue was observed by Tunel staining. Result:Compared with the model group, DZXYS was statistically significant in middle and high-dose group, and showed a notable tumor mass inhibition effect (P<0.05, P<0.01), improved tumor morphology, and increase in the number of cells in tumor tissues stained with Tunel and the tumor cell apoptosis rate (P<0.01). Conclusion:DZXYS can inhibit the growth of MCF-7 tumor tissue in nude mice, and its mechanism may be related to promoting tumor cell apoptosis.