当归贝母苦参丸含药血清对胃癌细胞SGC-7901抑制作用机制的研究

目的:研究不同浓度当归贝母苦参丸含药血清抑制胃癌细胞SGC-7901的机制。方法:将120只Wistar大鼠分为当归贝母苦参丸高、中、低剂量组(0.3,0.2,0.1 g·kg~(-1))和正常组,每组30只,ig 2次/d,连续给药7 d,制备含药血清,以空白血清处理胃癌细胞SGC-7901为空白组,实验组以当归贝母苦参丸含药血清高、中、低剂量组处理SGC-7901细胞后用RTq PCR技术检测环氧合酶-2(COX-2),Bcl-2相关X蛋白(Bax),人第10号染色体缺失的磷酸酶(PTEN)mRNA表达水平、用免疫细胞化学技术检测COX-2,BAX,PTEN蛋白表达情况。结果:与正常组比较,高剂量组使SGC-7901细胞COX-2 mRNA的表达量下降68%,BAX和PTEN mRNA的表达量分别升高99.7%和85.6%(P0.05),含药血清可以降低SGC-7901细胞COX-2的阳性表达率,升高其平均灰度值,升高BAX和PTEN的阳性表达率,减低其平均灰度值(P0.05)。结论:当归贝母苦参丸含药血清能抑制胃癌细胞SGC-7901,BAX,PTEN,COX-2 mRNA和蛋白的表达。

Effect of Danggui Beimu Kushen Wan Medicated Serum on Inhibition of Gastric Cancer Cells SGC-7901

Objective:To study the effect of Danggui Beimu Kushen Wan medicated serum on the inhibition of gastric cancer cells SGC-7901and its antineoplastic mechanisms. Method:The 120 Wistar rats were divided into Danggui Beimu Kushen Wan high dose group, middle dose group and low dose group (0.3, 0.2, 0.1 g·kg^-1) and normal group, n=30 in each group, ig 2 times/d, continuous for 7 days to prepare drug serum. The rats whose gastric cancer cells SGC-7901 were treated by blank serum were used as blank group. In the experiment groups, SGC-7901 cells were treated by high dose, middle dose and low dose Danggui Beimu Kushen Wan medicated serum, and then RT-qPCR technique was used to detect the expression level of mRNA of cyclooxygenase-2(COX-2), Bcl-associated X protein(Bax), and phosphatase and tensin homolog deleted on chromosome ten(PTEN). Immunocytochemical technique was used to test the protein expression level of COX-2, Bax, and PTEN. Result:Compared with the normal group, high dose group decreased the expression of COX-2 mRNA in SGC-7901 cell by 68%, and increased the expression of Bax and PTEN mRNA by 99.7% and 85.6% respectively (P<0.05). Drug serum can reduce the positive expression rate of COX-2 for SGC-7901 cells, increase its average gray value. increase the positive expression rate of Bax and PTEN, rate and reduce the average gray value (P<0.05). Conclusion:Danggui Beimu Kushen Wan medicated serum could inhibit the expressions of Bax, PTEN and COX-2 at mRNA and protein level in SGC-7901 gastric cancer cells.