| Heparanase antisense oligodeoxynucleotide inhibits angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice |
| |
| 作者姓名： | 张友磊 傅志仁 丁国善 张军 沈茜 王义 |
| |
| 作者单位： | [1]Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China [2]Liver Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China [3]Central Laboratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
| |
| 摘 要： | Objective： To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide （AS-ODN） on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods： The AS-ODN complementary to the start codon region of heparanase mRNA and its control, scrambled nonsense oligodeoxynucleotide （NS-ODN） were designed and synthesized. A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were established in nude mice and were treated with ODNs. The heparanase expression in tumor was evaluated by RT-PCR and Western blot. The microvessel density （MVD） was measured by immunohistochemistry for factor VS. The tumor volume was calculated and lung metastatic nodules were counted. Results ： The heparanase expression, MVD, tumor volume and lung metastatic nodules in AS-ODN treated group were significantly decreased compared with that in NS-ODN treated group and that in PBS group （P〈0.01）. Conclusion ： Heparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.
|
| 关 键 词： | 乳腺癌 血管发生 转移 裸鼠 动物实验 |
| 收稿时间： | 25 September 2006 |
| 修稿时间： | 2006-09-252007-05-10 |
Heparanase antisense oligodeoxynucleotide inhibits angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice |
| |
| ZHANG You-lei,FU Zhi-ren,DING Guo-shan,ZHANG Jun,SHEN Qian,WANG Yi.Heparanase antisense oligodeoxynucleotide inhibits angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice[J].Journal of Medical Colleges of PLA(China),2007,22(3):143-148. |
| |
| Authors: | ZHANG You-lei FU Zhi-ren DING Guo-shan ZHANG Jun SHEN Qian WANG Yi |
| |
| Institution: | 1. Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China; 2.Liver Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 3.Central Laboratory, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
| |
| Abstract: | ObjectiveTo evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide (AS-ODN) on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.MethodsThe AS-ODN complementary to the start codon region of heparanase mRNA and its control, scrambled nonsense oligodeoxynucleotide (NS-ODN) were designed and synthesized. A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were established in nude mice and were treated with ODNs. The heparanase expression in tumor was evaluated by RT-PCR and Western blot. The microvessel density (MVD) was measured by immunohistochemistry for factor VIII. The tumor volume was calculated and lung metastatic nodules were counted.ResultsThe heparanase expression, MVD, tumor volume and lung metastatic nodules in AS-ODN treated group were significantly decreased compared with that in NS-ODN treated group and that in PBS group (P<0. 01).ConclusionHeparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. |
| |
| Keywords: | heparanase antisense oligodeoxynucleotide mammary carcinoma angiogenesis metastasis |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
|
