鱼卵提取物微量刺激法诱导鼠脾细胞表达干细胞标志抗原

背景：小鼠和人的体细胞能被一些特殊的因子诱导,逆转为类似胚胎细胞的状态,即诱导性多能干细胞。目的：进一步提高鱼卵提取物诱导体细胞逆向分化为多能干细胞的效率。方法：以鱼卵提取物诱导BALB/C小鼠脾细胞分化为多能干细胞,分别以普通诱导法（鱼卵提取物质量浓度分别为0,0.1,0.2,0.4,0.8,1.2g/L）与微量刺激法诱导（鱼卵提取物质量浓度分别为0,0.05,0.1,0.2,0.4g/L）。结果与结论：与普通诱导法比较,微量刺激法诱导的脾细胞表达更多的干细胞标志抗原OCT-3/4,Nanog,SSEA-1。证明微量刺激法可进一步提高体细胞逆向分化为多能干细胞的效率。

A micro-stimulation method based on fish oocytes extracts induces mouse spleen cells to express stem cell mark antigen

BACKGROUND： The mouse and human somatic cells can be induced by some special factors to a state of embryonic cells called as induced pluripotent stem cells. OBJECTIVE： To further enhance the efficiency of fish oocytes extracts on inducing somatic cells to differentiate into pluripotent stem cells. METHODS： We compared a new method （micro-stimulation, 0, 0.05, 0.1, 0.2, 0.4 g/L fish oocytes extracts） to improve the induction efficiency, and the traditional method （0, 0.1, 0.2, 0.4, 0.8, 1.2 g/L fish oocytes extracts） in cultured cells by adding fish oocytes extracts. RESULTS AND CONCLUSION： Compared with the traditional method, the new micro-stimulation method could induce spleen cells to express more stem cell marker antigens OCT-3/4, Nanog, SSEA-1. It is proved that the micro-stimulation method is a more efficient way to generate pluripotent stem cells from somatic cells.