大鼠腭中缝牵张成骨中骨形态发生蛋白7的作用

背景：已有实验证实,骨形态发生蛋白7可诱导骨组织和软骨组织的形成,在牵张成骨的过程中促进骨组织形成。目的：测定骨形态发生蛋白7在腭中缝牵张成骨中的作用及机械扩张力作用下的变化。方法：采用自制双眼扩弓簧对大鼠施加扩张力,建立上颌骨扩张大鼠模型。分别于建模后第1,3,5,7,9,12,14天随机选取大鼠取腭中缝组织,实时定量PCR法测定目的基因的变化规律。结果与结论：建模后第3,9,12,14天大鼠腭中缝组织骨形态发生蛋白7mRNA的表达逐渐升高（P〈0.05）。结果证实,大鼠腭中缝牵张成骨时,骨形态发生蛋白7mRNA在机械力刺激下表达明显上调。

Role of bone morphogenetic protein-7 in the distraction osteogenesis of rat mid-palatal suture

BACKGROUND： Experiments have confirmed that bone morphogenetic protein-7 can induce bone and cartilage formation; it can also promote bone formation in the process of distraction osteogenesis. OBJECTIVE： To explore the role of bone morphogenetic protein-7 in the suture distraction osteogenesis of rat mid-palatal suture and to detect the changes of bone morphogenetic protein-7 under the mechanical expansion force. METHODS： Expansionary force was applied on rats using a self-made expansing arch spring for binoculus to construct maxillary expansion rat model. Rat mid-palatal sutures were randomly collected on 1, 3, 5, 7, 9, 12 and 14 days after model construction; real-time PCR was performed to detect the changing regularities of the target genes. RESULTS AND CONCLUSION： The expression of the bone morphogenetic protein-7 mRNA in the rat mid-palatal sutures increased gradually on 3, 9, 12 and 14 days after model construction （P 0.05）. These findings demonstrate that bone morphogenetic protein-7 mRNA expression is significantly increased in the distraction osteogenesis process of rat mid-palatal sutures under the stimulus of mechanical force.