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大鼠腭中缝牵张成骨中骨形态发生蛋白7的作用
引用本文:李威,李鹍,王培军,韩晶莹.大鼠腭中缝牵张成骨中骨形态发生蛋白7的作用[J].中国临床康复,2012(2):211-214.
作者姓名:李威  李鹍  王培军  韩晶莹
作者单位:[1]哈尔滨医科大学第二临床医学院口腔正畸科,黑龙江省哈尔滨市150086 [2]哈尔滨医科大学第二临床医学院科研科,黑龙江省哈尔滨市150086
基金项目:黑龙江省自然科学基金(D20965); 市科委青年科技创新人才基金资助项目(2010RFQXS113)~~
摘    要:背景:已有实验证实,骨形态发生蛋白7可诱导骨组织和软骨组织的形成,在牵张成骨的过程中促进骨组织形成。目的:测定骨形态发生蛋白7在腭中缝牵张成骨中的作用及机械扩张力作用下的变化。方法:采用自制双眼扩弓簧对大鼠施加扩张力,建立上颌骨扩张大鼠模型。分别于建模后第1,3,5,7,9,12,14天随机选取大鼠取腭中缝组织,实时定量PCR法测定目的基因的变化规律。结果与结论:建模后第3,9,12,14天大鼠腭中缝组织骨形态发生蛋白7mRNA的表达逐渐升高(P〈0.05)。结果证实,大鼠腭中缝牵张成骨时,骨形态发生蛋白7mRNA在机械力刺激下表达明显上调。

关 键 词:骨形态发生蛋白7  牵张成骨  大鼠  腭中缝  骨组织构建

Role of bone morphogenetic protein-7 in the distraction osteogenesis of rat mid-palatal suture
Li Wei,Li Kun,Wang Pei-jun,Han Jing-ying.Role of bone morphogenetic protein-7 in the distraction osteogenesis of rat mid-palatal suture[J].Chinese Journal of Clinical Rehabilitation,2012(2):211-214.
Authors:Li Wei  Li Kun  Wang Pei-jun  Han Jing-ying
Institution:1Department of Orthodontics, 2Research Division, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Abstract:BACKGROUND: Experiments have confirmed that bone morphogenetic protein-7 can induce bone and cartilage formation; it can also promote bone formation in the process of distraction osteogenesis. OBJECTIVE: To explore the role of bone morphogenetic protein-7 in the suture distraction osteogenesis of rat mid-palatal suture and to detect the changes of bone morphogenetic protein-7 under the mechanical expansion force. METHODS: Expansionary force was applied on rats using a self-made expansing arch spring for binoculus to construct maxillary expansion rat model. Rat mid-palatal sutures were randomly collected on 1, 3, 5, 7, 9, 12 and 14 days after model construction; real-time PCR was performed to detect the changing regularities of the target genes. RESULTS AND CONCLUSION: The expression of the bone morphogenetic protein-7 mRNA in the rat mid-palatal sutures increased gradually on 3, 9, 12 and 14 days after model construction (P 0.05). These findings demonstrate that bone morphogenetic protein-7 mRNA expression is significantly increased in the distraction osteogenesis process of rat mid-palatal sutures under the stimulus of mechanical force.
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