针灸预处理对兔心肌再灌注损伤心电图的影响

目的：研究针灸预处理内关穴对兔心肌再灌注损伤心电图的影响,为针灸防治心肌再灌注损伤提供实验依据.方法：将41只Wistar新西兰大耳白兔随机分为5组,采用冠脉结扎左前降支40 min、再灌注60 min,建立心肌梗死再灌注模型.冠脉结扎前予缺血预适应,电针、艾灸预处理内关穴.分别于结扎前、结扎40 min、再灌注60 min 3个时间点各记录心电图1次.结果：结扎后40 min,各处理组ST段和T波值均有不同程度的提高,与结扎前比较,差异有统计学意义（P＜0.01）,表明各组造模成功.再灌注60 min,T波值艾灸预处理组与结扎前比较,差异无统计学意义（P＞0.05）.ST段d1值：缺血预适应组与艾灸预处理组比较,差异有统计学意义（P＜0.05）,说明两组处理对ST段值改变有差异,在心梗发生时抑制ST段值抬高,缺血预适应组优于艾灸预处理组.ST段d2值：与假手术组比较,艾灸预处理组差异有统计学意义（P＜0.05）,余3组差异无统计学意义（P＞0.05）.T波d1值：与假手术组比较,4组差异均有统计学意义（P＜0.05或P＜0.01）.T波d2值：与假手术组比较,缺血预适应组及艾灸预处理组差异有统计学意义（P＜0.05或P＜0.01）,余2组无差异（P＞0.05）.结论：艾灸预处理内关穴及缺血预适应可降低心肌再灌注损伤程度,对心脏有保护作用.

Effect of Acupuncture and Moxibustion Preconditioning on ECG in Rabbit with Myocardial Reperfusion Injury

Objection] Objection：To study the influence of acupuncture and moxibustion preconditioning Neiguan on ECG in rabbitswith myocardial reperfusion injury,in order to offer experimental for acupuncture and moxibustion for treating myocardialreperfusion injury.Methods：The 41 Wistar New Zealand rabbits were randomly divided into five groups,and the left anteriordescending （LAD） coronary artery ligation for 40minutes and reperfusion for 60 minutes was applied to establish the reperfu-sion model of myocardial infarction.Electroacupuncture and moxibustion preconditioning on Neiguan was carried out before thecoronary artery ligation; the ECG was recorded,respectively before the coronary artery ligation as well as 40 minutes later and60 minutes after the reperfusion injury.Results：ST segment and T wave values increased at varying degrees in every treat-ment groups,40 minutes after the ligation,and the difference was statistically significant（P＜0.01）,which showed successfulmodel of myocardial infarction.The T-wave values in moxibustion pretreatment group,60 minutes after the reperfusion,showed no statistically significant difference（P＞0.05）; the difference of ST segment d1 value between ischemic preconditioninggroup and moxibustion pretreatment group was statistically significant（P＜0.05）,which showed the effect of the two methods onST segment was different and that the effect on inhibition of ST segment elevation in ischemic preconditioning group was bet-ter than the moxibustion pretreatment group; compared with the sham group,moxibustion pretreatment group showed statistical-ly significant difference on ST segment d2 values（P＜0.05）,and there was no difference between moxibustion pretreatment groupand the other three groups（P＞0.05）; compared with the sham group,the other four groups showed statistically significant differ-ence onT wave d1 value（P＜0.05 or P＜0.01）; compared with the sham group,ischemic preconditioning adaptation group andmoxibustion pretreatment group showed statistically significant difference on T wave d2 values（P＜0.05 or P＜0.01）,and the oth-er two groups showed no significant difference（P＞0.05）.Conclusion：Neiguan moxibustion pretreatment and ischemic precondi-tioning can reduce myocardial reperfusion injury,with a protective effect on the heart.