白藜芦醇对人皮肤T细胞淋巴瘤Hut-78细胞增殖和凋亡影响观察

目的：研究白藜芦醇对人皮肤T细胞淋巴瘤（cutaneous T cell lymphomas，CTCL）Hut78细胞株体外增殖和凋亡的影响，并初步探讨其作用机制。方法：用不同浓度的白藜芦醇（5、10和20μmol／L）作用于人皮肤CTCLHut-78细胞，24和48h后MTT检测白藜芦醇对细胞增殖的影响；TUNEL末端标记法和Annexin V—FITC双标记流式法检测白藜芦醇对细胞凋亡的影响。结果：经5、10和20μmol／L浓度的白藜芦醇处理24h后，细胞A值分别为1．202±0．094、0．568±0．019和0．535±0．033，与0μmol／LA值（1．272±1．107）比较，差异有统计学意义，P〈0．01。各浓度作用48h后，细胞A值分别为0．604±0．095、0．314±0．042和0．260±0．055，与0μmol／LA值（0．781±0．020）比较，差异有统计学意义，P〈0．01；与作用24h后同浓度A值比较，差异均有统计学意义，P〈0．01。随着时间和浓度的增加Hut-78细胞生长均受到不同程度的抑制，呈明显的浓度和时间依赖性。经5、lO和20／μmol／L浓度的白藜芦醇处理24h后，HUT-78细胞抑制率分别为（5．28±2．64）％、（53．52±10．86）％和（56．13±10．79）％，与0μmol／L抑制率（0）比较，差异均有统计学意义，P〈0．01；HUT-78细胞凋亡率分别为92．35％、96．39％和98．56％，与0μmol／L凋亡率（55．7％）比较，差异均有统计学意义，P〈0．01；各浓度作用48h后，HUT-78细胞抑制率分别为（22．85±10．98）％、（59．T7±5．44）％和（66．52±7．80）％，与0μmol／L细胞抑制率（O）比较，差异均有统计学意义，P〈0．01；与作用24h后同浓度细胞抑制率比较，差异均有统计学意义，P〈0．01。结论：达到5μmol／L的白藜芦醇对人皮肤T细胞淋巴瘤Hut-78细胞产生明显的抑制增殖作用，白藜芦醇主要通过诱导HuT-78细胞凋亡，其中主要是早期凋亡抑制其增殖。

Effect of resveratrol on inducing proliferation and apoptosis of T cell lymphoma cell line Hut-78 cells

OBJECTIVE：To investigate the effects of resveratrol on T cell lymphoma cell line Hut-78 cells and the possible mechanism by detecting the proliferation and apoptosis. METHODS： Twenty-four hours and 48 hours after the Hut-78 cells were treated with 5 μmol/L, 10μmol/L and 20 μmol/L resveratrol, the inhibitory effect was detected by MTT and the apoptosis was detected by PI staining and terminal deoxynucleotidy transferase-mediated dUTP nick end-labeling （TUNEL） assay. RESULTS：The A value was 1. 202±0. 094,0. 568±0. 019 and 0. 535±0. 033 with the treatment of different densities of resveratrol （5 μmol/L, 10 μmol/L,20 μmol/L） after 24 hours compared with the control group （1. 272±0. 1070,P〈0.01）. The increasing of time and density decreased the viability of Hut 78 cells in a density and time-dependent manner. 24 hours after the Hut-78 ceils treated with 5 μmol/L, 10 μmol/L and 20 μmol/L resveratrol,the rate of inhibitory were respectively （5.28±2.64）%,（53.52±10.86）% and （56.13± 10.97）%（P〈0.01）,and the rate of apoptosis were respectively 92.35 %, 96.39 %, 98.56 % （P〈0.01）. Forty eight hours after the Hut-78 cells treated with 5 μmol/L,10 μmol/L and 20 /μmol/L resveratrol,the rate of inhibitory were respectively （22.85± 10. 98）%, （59.77± 5.44）% and （66.52± 7.80） % （P〈0.01）. CONCLUSION： The level of resveratrol reaches to 5 μmol/L or higher can inhibit the proliferation of Hut-78 cells, mostly through inducing apoptosis, especially early apoptosis.