| 毛细管区带电泳测定血清胸腺因子的方法 |
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| 引用本文： | 曲宁,陈竞,夏弈明. 毛细管区带电泳测定血清胸腺因子的方法[J]. 卫生研究, 2001, 30(5): 313-314 |
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| 作者姓名： | 曲宁 陈竞 夏弈明 |
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| 作者单位： | 中国预防医学科学院营养与食品卫生研究所, |
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| 摘 要： | 采用区带电泳测定血清胸腺因子 (FTS) ,通过正交设计 ,对缓冲液种类、盐离子浓度及pH值进行了筛选 ,其最佳实验方案为 0 0 5mol L硼砂 ,0 0 1mol L磷酸盐缓冲液 ,pH8 70 ,电压 12kV ,温度 2 5℃ ,检测波长2 0 0nm ,用区带电泳能达到较好分离。对标准样品连续 5次测定的相对标准差为 7 6 2 %。对牛血清白蛋白和血清样品分别加入FTS标准 ,其回收率为 80 2 8% ,检出限为 40ng ml。
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| 关 键 词： | 毛细管电泳 血清胸腺因子 正交设计 |
| 文章编号： | 1000-8020(2001)05-0313-02 |
| 修稿时间： | 2000-10-15 |
Studies on the determination of serum thymic factor by capillary zone electrophoresis |
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| Qu Ning,Chen Jing,Xia Yiming Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine,Beijing ,China. Studies on the determination of serum thymic factor by capillary zone electrophoresis[J]. Journal of hygiene research, 2001, 30(5): 313-314 |
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| Authors: | Qu Ning Chen Jing Xia Yiming Institute of Nutrition Food Hygiene Chinese Academy of Preventive Medicine Beijing China |
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| Affiliation: | Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China. |
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| Abstract: | The determination of serum thymic factor (FTS) by capillary zone electrophoresis was described. The optimum ionic concentration and the pH value of buffer system was examined through orthogonal analysis.The optimum conditions found to be 0 05mol/L sodium tetraborate,0 01mol/L phosphate buffer at pH 8 70,working voltage at 12kV and wavelength at 200nm. The recoveries for FTS standards added to the bovine serum albumin and serum samples were measured.The values of the relative standard deviation(RSD),recovery and detection limit were 7 62%,80 28% and 40ng/ml respectively.The development of this method provide another way for studying the relations of zinc and thymic factor and investigating the effect of zinc on immune function. |
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| Keywords: | capillary zone electrophoresis serum thymic factor othogonal analysis |
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