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Neurons and stromal stem cells as targets for polycation-mediated transfection |
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| Authors: | Shakhbazau A V Shcharbin D G Goncharova N V Seviaryn I N Kosmacheva S M Kartel N A Bryszewska M Majoral J P Potapnev M P |
| Affiliation: | (1) Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Akademicheskaya, 27, 220072 Minsk, Belarus;(2) Institute of Biophysics and Cellular Engineering, National Academy of Sciences of Belarus, Akademicheskaya, 27, 220072 Minsk, Belarus;(3) Republic Centre for Haematology and Transfusiology, Minsk, Belarus;(4) Laboratory of Electron Microscopy, University of Lodz, Lodz, Poland;(5) Department of General Biophysics, University of Lodz, Lodz, Poland |
| Abstract: | Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher. |
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