MBP-52位点多态性与肺结核易感性的病例对照研究

[目的]探讨甘露糖结合蛋白(MBP)基因多态性与汉族人群肺结核发病的关系。[方法]采用病例对照研究,用特异引物PCR方法进行MBP的基因分型,对肺结核发病影响因素进行问卷调查,并进行单因素分析和多因素非条件Logistic回归分析。[结果]对126例病例和202例对照的MBP基因52位点进行分型,野生型、突变型(杂合子 纯合子)两种基因型在病例组和对照组中的分布频率分别为男性86.7%、13.3%、95.7%和4.3%;女性93.0%、7.0%、85.9%和14.1%。男性病例的突变频率显著高于对照组(OR为6.222,OR95%CI为1.285～30.134),而女性病例的突变频率明显低于对照组(OR为0.646,OR95%CI为0.564～0.740)。在多因素分析中调整卡介苗接种史、卡痕和家族史后,MBP-52基因突变与肺结核发病仍有统计学意义,与单因素分析的结果相同。[结论]MBP-52位点C/T突变可能与肺结核发病相关。

A CASE CONTROL STUDY ON MANNOSE-BINDING PROTEIN GENETIC POLYMORPHISM AND SUSCEPTIBILITY TO PULMONARY TUBERCULOSIS

[Objective]To investigate the association between the genetic polymorphism of MBP gene and susceptibility to pulmonary tuberculosis.[Methods]A case-control study was conducted. Polymerase chain reaction with sequence-specific primers(PCR-SSP)was carried out in the polymorphism for MBP gene. The information on related factors of tuberculosis was collected with questionnaire interview. Univariate and multivariate Logistic analyses were conducted.[Results]126 cases and 202 controls were analyzed,the frequency of wildness?mutant gene(heterozygote and homozygote)were 86.7%,13.3%,95.7% and 4.3% for males;93.0%,7.0%,85.9% and 14.1% for females respectively. Mutant gene homozygote and heterozygote occurred more frequently in the cases than controls for males,genotype occurred MBP-52-C/T or T/T with crude OR(95%CI)being 6.222(1.285-30.134),but it was less frequent in the cases than controls for females with crude OR(95%CI)being 0.646(0.564-0.740). The significant association remained after adjusting three related environmental factors(vaccinal vestige,BCG immunization and familial traits)in the multivariate Logistic analyses.[Conclusions]MBP-52 genotype C/T might be involved into the susceptibility to pulmonary tuberculosis.