| 淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析 |
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| 引用本文： | 贺超,廖芳,刘海鹏,刘丽江. 淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析[J]. 中国妇幼保健, 2006, 21(11): 1524-1525 |
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| 作者姓名： | 贺超 廖芳 刘海鹏 刘丽江 |
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| 作者单位： | 1. 华中科技大学同济医学院病原生物学系,湖北,武汉,430030
2. 华中科技大学同济医学院同济医院妇产科 |
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| 基金项目： | 湖北省卫生厅资助项目(No.JX1B037) |
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| 摘 要： | 目的:构建淋病奈瑟菌孔蛋白PIA(Porin IA)基因的真核表达系统,为淋病DNA疫苗的研究提供材料。方法:PCR技术扩增淋病奈瑟菌捷克标准株的PIA基因片段,将其定向插入真核表达载体pC I-neo多克隆位点中,构建重组表达载体。并用酶切分析、PCR扩增及序列测定方法,对重组载体进行鉴定。结果:淋病奈瑟菌PIA基因被正确克隆到真核表达载体pC I-neo中,测序结果与Genebank中AF090819等菌株序列有99.99%同源性。结论:真核表达系统(pC I-PIA)构建成功。
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| 关 键 词： | 淋病奈瑟菌 PIA基因 真核表达 |
| 文章编号： | 1001-4411（2006）11-1524-02 |
| 修稿时间： | 2006-04-29 |
Construction of An Eukaryotic Expression System pCI -PIA Carrying the Porin IA Gene from the Neisseria Gonorrhoeae and Sequence Determination |
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| HE Chao,LIAO Fang,LIU Hai-Peng,LIU Li-Jiang. Construction of An Eukaryotic Expression System pCI -PIA Carrying the Porin IA Gene from the Neisseria Gonorrhoeae and Sequence Determination[J]. Maternal and Child Health Care of China, 2006, 21(11): 1524-1525 |
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| Authors: | HE Chao LIAO Fang LIU Hai-Peng LIU Li-Jiang |
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| Affiliation: | Department of Microbiology, School of Basic Medical Science, Tong ji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective:To construct an eukaryotic expression system carrying the Neisseria gonorrhoeae Porin IA(PIA)gene.Methods:The PIA gene was amplified from the genome of the Neisseria gonorrhoeae by PCR and recombined with plasmid pCI-neo.The recombinant plasmid pCI-PIA was verified with restriction analysis,PCR and sequence.Results:PIA gene was cloned correctly into vector pCI-neo.Conclusion:The eukaryotic expression system pCI-PIA was constructed successfully. |
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| Keywords: | Neisseria gonorrhoeae Porin IA gene Eukaryotic expression DNA vaccine |
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