灵芝水提取物对人免疫细胞及IL-6的调节作用

目的：观察灵芝水提取物对人免疫细胞及细胞因子的调节作用。方法：灵芝水提取物（ＧＬ－Ｗ）作用于人外周血淋巴细胞（ＰＢＬ），用ＭＴＴ法及流式细胞仪技术检测。结果：１．ＧＬ－Ｗ以经植物凝集素（ＰＨＡ）活化的和未经ＰＨＡ活化的人ＰＢＬ均有促增殖效应，其中经ＰＨＡ活化的增殖反应显著（Ｐ〈０．０５），细胞周期检测发现其主要促进细胞进入Ｓ期，表明ＧＬ－Ｗ可促进人ＰＢＬ的ＤＮＡ合成。２．选择最大促进增殖效诮浓度的

Regulation Effect of Water-extraction from Ganoderma Lucidum (Lyess.ex Fr) karst. on Human Immune cells and IL-6

Objective:To study the regulation effect of water extraction from Ganoderma Lucidum (Lyess. ex Fr) karst (GL W) on human immune cells and cytokine. Methods: With the technologies of MTT and FACS, the proliferation of T lymphocyte subgroup and IL 6 production in human peripheral blood lymphocytes (PBL) were studied. Results: 1. In the absence of mitogin, GL W could induce not only the inactive lymphocyte but also the PHA activited lymphocyte to proliferate in the similar concentration. The proliferation in the later was more obvious ( P <0.05). The cell cycle analysis found that the increment was mainly at the stage of S phase, which indicated that GL W could promote the DNA synthesis of human PBL. 2. It was found that GL W could induced the CD4 cell ratio to increase during the double label detection of PHA activited T lymphocyte subgroup added by the maximum proliferation promoting effect concentration of GL W. This indicated that GL W had the immunity promoting effect. 3. GL W could have the induced production promoting effect of IL 6 on the activited human PBL. Conclusions: GL W can enhance the immune function of human body.