| Notch-Dll4在激光诱导的大鼠脉络膜新生血管生成中的作用 |
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| 引用本文： | 马可,李晓,王雨生,叶子,蔡岩. Notch-Dll4在激光诱导的大鼠脉络膜新生血管生成中的作用[J]. 眼科新进展, 2010, 30(5) |
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| 作者姓名： | 马可 李晓 王雨生 叶子 蔡岩 |
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| 作者单位： | 第四军医大学西京医院眼科、全军眼科研究所,陕西省西安市,710032 |
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| 基金项目： | 国家自然科学基金资助(编号:30872818)~~ |
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| 摘 要： | 目的探讨Notch信号通路中Delta样配体4(Delta-like ligand4,Dll4)在激光诱导的大鼠脉络膜新生血管(choroidal neovascularization,CNV)生成中的作用机制。方法将雄性棕色挪威(brown-Norway,BN)大鼠分为正常组、光凝组、实验组和对照组。光凝组、实验组、对照组利用532nm激光诱导建立双眼CNV模型。实验组在建立CNV模型后立刻玻璃体内注射25g.L-1Avastin10μL,对照组注射0.01mmol.L-1PBS10μL。采用免疫荧光染色检测Dll4和血管内皮生长因子(vascular endo-thelial growth factor,VEGF)在正常组和光凝组光凝后7d中的表达;采用Western-blotting和RT-PCR检测正常组和光凝组(光凝后1d、3d、7d、14d)实验组、对照组(注射后7d)Dll4和VEGF的蛋白和mRNA表达;通过HE染色和视网膜色素上皮-脉络膜-巩膜铺片检测实验组和对照组(注射后14d)CNV生成的厚度和面积。结果Dll4和VEGF在CNV区域有共表达。Dll4和VEGF蛋白及其mRNA的表达趋势...
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| 关 键 词： | Delta样配体4 血管内皮生长因子 脉络膜新生血管 |
Effect of Notch-Dll4 in laser-induced choroidal neovascularization in rats |
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| MA Ke,LI Xiao,WANG Yu-Sheng,YE Zi,CAI Yan From the. Effect of Notch-Dll4 in laser-induced choroidal neovascularization in rats[J]. Recent Advances in Ophthalmology, 2010, 30(5) |
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| Authors: | MA Ke LI Xiao WANG Yu-Sheng YE Zi CAI Yan From the |
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| Affiliation: | MA Ke,LI Xiao,WANG Yu-Sheng,YE Zi,CAI Yan From the Department of Ophthalmology,Eye Institute of Chinese PLA,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China |
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| Abstract: | Objective To investigate the effective mechanism of Delta-like ligand 4(Dll4)in Notch signal pathway in choroidal neovascularization(CNV).Methods Brown-Norway(BN)rats were randomly divided into four groups,including normal group,photocoagulation group,treatment group and control group.The experimental CNV in double eyes was induced by 532 nm laser photocoagulation in rats of photocoagulation group,treatment group and control group.As soon as photocoagulation,the rats in treatment group were treated by intra... |
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| Keywords: | Delta-like ligand 4 vascular endothelial growth factor choroidal neovascularization |
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