BALB/c小鼠噁唑酮结肠炎模型的建立

目的:建立BALB/c小鼠噁唑酮结肠炎模型,评价其作为人类溃疡性结肠炎(UC)模型的可靠性.方法:模型组第1天和第2天用3％噁唑酮溶液200 μL涂搽BALB/c小鼠皮肤以致敏,第6天用1％噁唑酮溶液150μL行保留灌肠,对照组给予等体积乙醇溶剂涂搽皮肤、灌肠,对2组BALB/c小鼠进行疾病活动指数(DAI)评分,评估病变结肠的病理组织学炎症程度,用荧光定量逆转录聚合酶链反应(RT-PCR)法测定结肠组织IL-5和IL-13 mRNA的表达水平.结果:模型组小鼠的DAI评分和病理炎症评分显著高于对照组(P＜0.05),模型组病变结肠组织IL-5和IL-13 mRNA的表达水平亦显著高于对照组(P＜0.05).结论:BALB/c小鼠噁唑酮结肠炎模型类似于人类UC,可作为研究UC的理想模型.

Establishment of BALB/c Mice Oxazolone-Colitis Model

Objective：To establish BALB/c mice oxazolone-colitis model and evaluate its reliability as a model of human ulcerative colitis（UC）.Methods：At day 1 and day 2 model group mice were skin-sensitized with 200μl 3%oxazolone,and at day 6 150μl 1%axazolone was administered intrarectally.Control group mice were skin-sensitized and intrarectally administered with equal-volume solvent of ethanol.We observed the disease activity index（DAI） of th two group,evaluated the histopathological inflammation degree of the lesions of the colon,and determined the expression levels of IL-5 and IL-13 mRNA of the colonic tissue by real time polymerase chain reaction（RT-PCR）.Results：The DAI score and the histopathological inflammation score of the model group were significantly greater than that of the control group,and the expression levels of IL-5 and IL-13 mRNA of the colonic tissue of the model group were also significantly higher than that of the control group.Conclusions：BALB/c mice oxazolone-colitis model is resemble to human UC,and could be used as an ideal research platform of UC.