组织型转谷氨酰胺酶和血管内皮生长因子在上皮性卵巢癌中的表达及其意义

目的探讨组织型转谷氨酰胺酶（tTG）和血管内皮生长因子（VEGF）在上皮性卵巢癌中的表达及其与卵巢癌侵袭、转移的相关性。方法应用免疫组化SP法对55例上皮性卵巢癌组织（卵巢癌组）、21例良性卵巢肿瘤组织（卵巢良性肿瘤组）中tTG、VEGF的表达情况进行检测，并分析其与临床病理指征的关系及二者表达的相关性。结果tTG、VEGF在上皮性卵巢癌组织和卵巢良性肿瘤中的阳性表达率分别为92．7％（51／55）、38．1％（8／21）及90．9％（50／55）、47．6％（10／21），上皮性卵巢癌中的表达明显高于卵巢良性肿瘤组织，差异有统计学意义（χ^2值分别为32．620、19．570，P均〈0．01）。tTG在上皮性卵巢癌组织中的表达与临床分期（χ^2=13．286，P〈0．01）、有无腹水（χ^2=17．247，P〈0．01）有关；与病理学类型、组织学分级及淋巴结转移无关（P均〉0．05）。VEGF在上皮性卵巢癌组织中的表达与临床分期（χ^2=17．542，P〈0．01）、组织学分级（χ^2=18．720，P〈0．01）及有无腹水（χ^2=22．037，P〈0．01）有关；与病理学类型、淋巴结转移无关（P均〉0．05）。tTG在上皮性卵巢癌基质细胞中的表达亦与临床分期（χ^2=11．250，P〈0．01）、有无腹水（χ^2=12．406，P〈0．01）有关；与病理学类型、组织学分级及淋巴结转移无关（P均〉0．05）。VEGF在上皮性卵巢癌基质细胞中的表达与各项临床病理指标均无关（P均〉0．05）。上皮性卵巢癌组织中tTG蛋白表达与VEGF阳性表达呈显著相关（r=0．8856，χ^2=43．14，P〈0．01）。结论tTG、VEGF共同表达在上皮性卵巢癌的发生、发展中起重要作用，二者的相互协同作用可能是促进卵巢癌发生、浸润和转移的重要因素。

Expression and significance of tissue transglutaminase and VEGF in epithelial ovarian carcinoma

Objective To investigate the expression of tissue transglutaminase （tTG） and vascular endothelial growth factor（VEGF） in epithelial ovarian carcinoma and the relationship of invasion and metastance in ovarian carcinoma. Methods The expression of tTG and VEGF was detected in 55 malignant and 21 benign ovarian neoplasms by immunohistochemical SP method. The clinical significance of tTG and VEGF and their relationship were analyzed. Results The expression rates of tTG and VEGF in malignant epithelial ovarian tumors were significandy higher than in benign epithelial ovarian tumors （tTG ：92. 7% vs. 38. 1% , χ^2 = 32. 620, P 〈0. 01 ;VEGF：90. 9% vs. 47. 6% ,χ^2 = 19. 570,P 〈0.01 ）. In ovarian cancer,the expression of tTG in tumor cells and stromal cells was significandy related to clinical phases and the presence of ascites. The expression of VEGF in tumor cells had a significant revelance to clinical phases, pathological grades and ascites. The expression of tTG in ovarian cancer cells was positively correlated with VEGF （ r = 0. 8856, χ^2 = 43.14, P 〈 0. 01 ）. Conclusion Tissue transglutaminase and VEGF were detected not only in tumor cells, but also in stromal cells. High expression of tTG and VEGF may play an important role in the pathogenesis of ovarian cancer and their synergistic effect may act as an important factor in promoting the invasion and metastasis of this cancer.