硝基酪氨酸与2型糖尿病冠心病的关系研究

目的观察血清硝基酪氨酸水平与2型糖尿病（T2DM）冠心病（CHD）的关系。方法应用ELISA免疫试剂盒测定109例糖尿病非冠心病患者（T2DM组）、152例糖尿病合并冠心病患者（T2DM-CHD组）和103名正常对照者（对照组）硝基酪氨酸浓度。结果（1）T2DM-CHD组血清硝基酪氨酸浓度为（78．17±10．68）nmol／L，高于T2DM组（70．50±9．13）nmol／L]及对照组（63．23±11．55）nmol／L]，差异均有统计学意义（P均〈0．01）。（2）对所有糖尿病患者进行相关分析发现，硝基酪氨酸与总胆固醇、甘油三酯、空腹血糖、Gensini积分相关（r=0．361，P=0．009；r=0．206，P=0．001；r=0．347，P=0．026；r=0．466，P〈0．001）。Logistic回归分析发现硝基酪氨酸是2型糖尿病合并冠心病的危险因素之一（OR=1．094，95％CI：1．053-1．137；P〈0．01）。结论硝基酪氨酸在2型糖尿病及2型糖尿病合并冠心病的发生过程中起重要作用。

Association between nitrotyrosine and coronary heart disease in type 2 diabetes

Objective To investigate the association of nitrotyrosine with coronary heart disease （CHD） in type 2 diabetes mellitus. Methods The nitrotyrosine levels were determined in 109 patients of type 2 diabetes mellitus without CHD （T2DM）. One hundred and fifty-two patients of type 2 diabetes mellitus with CHD （T2DM-CHD） and 103 healthy control subjects by ELISA. Results T2DM-CHD patients had significantly increased nitrotyrosine compared with T2DM group and the control group （ 78.17±10.68 ） nmol/L, （70. 50±9. 13） nmol/L vs （63.23±11.55 ） nmol/L, Ps 〈 0. 01 ]. Nitrotyrosine was correlated with total cholesterol, triglyceride, fasting glucose and Gensini Score （ r = 0. 361, P = 0. 009 ; r = 0. 206, P = 0. 001 ; r = 0. 347, P = 0. 026 ; r = 0. 466, P 〈 0. 001 ） . Multivariable logistic regression showed nitrotyrosine was independently associated with CHD combined with type 2 diabetes meUitus （ OR = 1. 094,95% CI： 1. 053 - 1. 137 ;P 〈 0. 01 ）. Conclusion Nitrotyrosine plays an important role in the formation and development of cardiovascular disease in tvne 2 diabetes.