p16在尖锐湿疣和鲍温样丘疹病中的表达

目的:检测细胞周期相关蛋白p16在尖锐湿疣(condyloma acuminatum,CA)和鲍温样丘疹病(bowenoid papulosis,BP)病变组织中的表达情况,探讨p16在鉴别诊断CA与BP中的意义,推测其在CA与BP向不典型增生及肿瘤转化的可能作用.方法:收集外阴病理活检确诊为CA与BP患者的病变组织标本,采用免疫组织化学Envision法、IMS细胞图象分析系统分析p16蛋白表达,以阳性细胞数百分比为结果,并与正常包皮组织对照.结果:28例病理确诊为CA患者的病变组织,p16阳性细胞数百分比为41.330％±3.823％；28例病理确诊的BP患者的病变组织,p16阳性细胞数百分比为55.520％±3.638％；对照组10例正常包皮组织,p16阳性细胞数百分比为26.990％±0.807％,差异有统计意义(P＜0.05).结论:CA和BP病变组织中存在p16的异常表达,人乳头状瘤病毒(HPV)可能通过作用于细胞周期相关蛋白导致细胞周期紊乱,继而导致非典型增生的发生,其确切机制尚待进一步研究.

Research on Expression of p16 in Condyloma Acuminatum and Boweniod Papulosis

Objective：To investigate the expression of pl6 in condyloma acuminatum（CA）and bowenoid papulosis（BP）.Methods： Preserved the specimens of CA,BP and normal prepuce tissue used as control by paraffin imbedding.Envision immunohis-tology technology with IMS（image analysis system） was applied to detect the expression of pl6.Results：In the 28 specimens of CA,the percentage of p16 positive cells was 41.330%±3.823%.In the 28 specimens of BP,the percentage of p16 positive cells was 55.520%±3.638%,while the result was 26.990%±0.807%respectively in the 10 controlled prepuce tissues.The difference was statistically significant（P〈0.05）.Conclusions：The expression of p16 was elevated and independence in CA and BP,suggesting the derangement of cell cycle.