| 高效毛细管电泳法测定蒲公英及妇洁灌肠液中咖啡酸的含量 |
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| 摘 要： |
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| 关 键 词： | 高效毛细管电泳法 测定 蒲公英 妇洁灌肠液 咖啡酸 含量 |
High-performance capillary electrophoresis for determining caffeic acid content in Fujie enema and Taraxacum mongolicum Hand.-Mazz] |
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| Yuan Yan,Zhong-yuan Xu,Zhi-liang Chen,Ya-fei Tan,Dan Guo. High-performance capillary electrophoresis for determining caffeic acid content in Fujie enema and Taraxacum mongolicum Hand.-Mazz][J]. Journal of First Military Medical University, 2004, 24(4): 456-458 |
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| Authors: | Yuan Yan Zhong-yuan Xu Zhi-liang Chen Ya-fei Tan Dan Guo |
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| Affiliation: | Department of Pharmaceutics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To determine caffeic acid content in Fujie enema and the crude traditional Chinese herbal drug Taraxacum mongolicum Hand.-Mazz. for controlling the quality of the enema at the levels of both the crude drugs and the final product. METHODS: Caffeic acid content in both the enema and the crude drug was determined at 313 nm Using high-performance capillary electrophoresis (HPCE), under the optimized conditions achieved with a fused-silica capillary tube (75 micromx0 cm) and 20 mmol/L borate running buffer (pH=9.18) at a constant voltage of 12 kV and a sampling time of 5 s at 25 degrees Celsius. RESULTS: The calibration curve displayed good linear relationship within caffeic acid concentration range of 10 to 100 microg/ml (r=0.999 2). The regression equation was Y=1002.45X-327.87, and the average recovery was more than 95%. CONCLUSION: HPCE is simple, rapid and sensitive in separation and determination of caffeic acid in Fujie enema and the crude drug of Taraxacum mongolicum Hand.-Mazz. |
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