Interpore 500R复合人骨髓基质干细胞构建组织工程骨

目的：研究可吸收性珊瑚羟基磷灰石（Interpore 500R）作为骨组织工程支架材料的特点。方法：取人骨髓基质干细胞于含100ml/L胎牛血清的DMEM培养液中培养，并向成骨细胞诱导。将细胞与可吸收性珊瑚羟基磷灰石复合，通过扫描电镜观察细胞附着情况，共聚焦显微镜观察I型胶原的分泌。同时将细胞/Interpore500R复合物植入裸鼠体内构建骨组织。40d后取裸鼠标本组织切片、HE染色观察成骨情况。结果：骨髓基质干细胞在可吸收性羟基磷灰石表面附着良好，可分泌I型胶原，在裸鼠体内形成组织工程骨。结论：可吸收性羟基磷灰石生物相容性好，细胞附着后仍继续保持成骨细胞特性，并可形成骨组织。作为骨组织工程的支架材料，应用前景良好。

Absorbable coral hydroxyapatite as a scaffold for tissue engineered bone in vitro

?Objective:To investigate the behavior of absorbable coral hydroxyapatite (Interpore 500R) as bone tissue engineering scaffold. Methods: Marrow stromal stem cells harvested from legally aborted human fetus were cultured in DMEM containing 100 ml/L fetal boving serum and induced to differentiate towards osteoblasts.Then the cells were seeded onto absorbable coral hydroxyapatite , then the composite was implanted subcultaneously into the back of nude mice .Cell materia attachement was observed with scanning electron microscope,type I collegen secretion was studied with laser confocal microscope and new bone formation was studied histologicaley. Results: The cells could adhere to absorbable coral hydroxyapatite and secrete collagen I.Mature bone tissue was found in the implanted composite 40 day after transplantation. Conclusion: Absorbable coral hydroxyapatite Interpore 500R is a favorable bone tissue engineering scaffold.