43株临床铜绿假单胞菌exoS、exoU基因的携带及其耐药性 FREE

目的 研究43株临床分离的铜绿假单胞菌Ⅲ型分泌系统毒素基因exoS、exoU携带情况以及细菌的耐药性。方法 采用聚合酶链反应（PCR）法检测毒素基因的分布，K B纸片扩散法检测细菌的耐药性。结果 43株临床铜绿假单胞菌中，37株(86.05%)exoS基因阳性，6株(13.95%)exoU基因阳性，无同时携带2种基因菌株。携带exoU的临床株对9种抗菌药物的耐药率均高于携带exoS菌株，其中对头孢他啶、环丙沙星、左氧氟沙星的耐药率差异有显著性（P＜0.05）。结论 携带毒素基因exoU的铜绿假单胞菌临床株所占比例较低，但耐药率高。

Carrying of exoS and exoU in 43 clinical isolates of Pseudomonas aeruginosa and their drug resistance

Objective To study the carrying of type III secretion systems toxin gene exoS and exoU in 43 clinical isolates of Pseudomonas aeruginosa （P. aeruginosa） and drug resistance. Methods The distribution of toxin gene was detected by PCR, antimicrobial resistance of clinical strains was detected by Kirby-Bauer method. Results Each P. aeruginosa isolate contained either exoS or exoU gene, exoS and exoU was harbored by 37（86. 05 %） and 6 （13.95 %） isolates respectively. Resistant rates of exoU-harbouring isolates to 9 kinds of antimicrobial agents were higher than exoS-harbouring isolates, and there were significant difference in resistance to ceftazidime, ciprofloxacin and levofloxacin between exoU- and exoS-harbouring isolates （P〈0. 05）. Conclusion exoU-harbouring isolates are far less than exoS-harbouring isolates, and exoU-harbouring isolates appear high drug-resistance.