Prenylated isoflavones from Cudrania tricuspidata inhibit NO production in RAW 264.7 macrophages and suppress HL-60 cells proliferation |
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Authors: | Hoang Le Tuan Anh Do Thanh Tuan Do Thi Trang Bui Huu Tai Nguyen Xuan Nhiem Pham Hai Yen |
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Affiliation: | 1. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, Hanoi, Vietnam;2. Department of Biology, Thaibinh Medical University, Thaibinh, Vietnam |
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Abstract: | Inhibitory effects of NO production in RAW 264.7 macrophages guided the isolation of nine prenylated isoflavones, including a new cudraisoflavone L (1) and eight known metabolites furowanin B (2), erysubin A (3), wighteone (4), lupalbigenin (5), laburnetin (6), isolupalbigenin (7), 6,8-diprenylorobol (8), millewanin H (9) from the leaves of Cudrania tricuspidata. At the concentration of 10 μM, compounds 1, 2, and 4 significantly inhibited NO production with the inhibitory values of 72.5 ± 2.4, 66.9 ± 1.8, and 55.4 ± 2.7%, respectively. In addition, all of isolated compounds 1–9 showed promising cytotoxic effects toward HL-60 cells (IC50 4.3 ± 0.7 to 18.0 ± 1.7 μM). |
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Keywords: | Prenylated isoflavone cudraisoflavone L Cudrania tricuspidata NO inhibitor anti-leukemia |
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