多发性骨髓瘤患者MMP-2及VEGF表达的研究

 目的 探讨基质金属蛋白酶-2（ MMP-2）、血管内皮细胞生长因子（VEGF）水平与多发性骨髓瘤（MM）的病情进展的关系和临床意义。方法 用酶联免疫吸附试验（ELISA）分别检测了24例MM患者和10例正常对照骨髓基质细胞（BMSC）培养上清液的MMP-2、VEGF水平。结果 14例进展期MM组的MMP-2、VEGF表达水平显著高于10例稳定期MM组和10例正常对照组（P＜0.05），稳定期MM组和正常对照组的MMP-2水平差异无统计学意义（P＞0.05），稳定期MM组VEGF水平高于正常对照组（P＜0.05）。9例进展期MM组的BMSC与人骨髓瘤细胞系细胞U266（终浓度2×105／ml）进行混合培养48 h后，其上清中MMP-2、VEGF水平明显增高，显著高于进展期MM、稳定期MM、正常对照组BMSC及稳定期MM BMSC + U266组的水平（均为P＜0.05）。稳定期MM BMSC + U266 组的VEGF水平显著高于进展期MM、稳定期MM、正常对照组BMSC（P＜0.05），差异有统计学意义。MMP-2与VEGF两者呈正相关（r =0.925，P＜0.05）。结论 MMP-2、VEGF水平升高与MM的病情进展或发病有关，该过程可能需骨髓瘤细胞和骨髓基质细胞协同作用而完成。

Studies of MMP-2 and VEGF expression in multiple myeloma patients

Objective To explore the role and implication of matrix metalloproteinase-2(MMP-2) and vascular endothelial growth factor （VEGF）in multiple myeloma (MM). Methods Expression MMP-2 and VEGF secreted by bone marrow stromal cells(BMSC ) of 24 MM patients were detected by ELISA. Results The level of MMP-2 and VEGF in 14 active MM patients were significantly higher than 10 stable MM pa－tients and 10 healthy subjects(P <0.05), The level of VEGF in stable MM patients were different from healthy subjects(P <0.05) When human MM cell lines U266 were adhered to BMSC of 9 active MM patients. The level of MMP-2 and VEGF in active and stable MM patients were significantly higher than BMSC alone(P <0.05). The expression level of MMP-2 was related to VEGF（r =0.925, P <0.05）. Conclusion The increase of MMP-2 and VEGF activity produced by BMSC or induced by myeloma cells in these cocultures is associated with progress or pathogenesis of MM, and may be invoved with tumor invasion.