| 火焰原子吸收分光光度法测定SD大鼠全血之钙镁铁锌铜 |
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| 引用本文： | 王小平,朱奇,季莘,丁亦青,李春建,高超. 火焰原子吸收分光光度法测定SD大鼠全血之钙镁铁锌铜[J]. 南通大学学报(医学版), 2012, 32(4): 253-255 |
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| 作者姓名： | 王小平 朱奇 季莘 丁亦青 李春建 高超 |
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| 作者单位： | 1. 南通大学公共卫生学院,南通,226019
2. 南通大学杏林学院,南通226019;南通大学公共卫生学院,南通226019 |
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| 基金项目： | 南通大学杏林学院自然科学研究课题 |
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| 摘 要： | 目的:用火焰原子吸收分光光度法测定正常SD大鼠全血中钙、镁、铁、锌、铜的含量,建立正常SD大鼠全血中此5种元素的正常值范围.方法:使用浓硝酸和高氯酸(4:1混合酸)对SD大鼠全血进行加热消解,用火线原子吸收标准曲线法测定全血中的钙、镁、铁、锌、铜含量,并做加标回收实验.结果:采用此方法回收率为90％～110％,用此方法测定建立的正常SD大鼠全血中钙、镁、铁、锌、铜的正常值范围稳定,具有可信性.结论:本方法操作简单、测定值稳定,结果令人满意.
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| 关 键 词： | 火焰原子吸收分光光度法 全血 钙 镁 铁 锌 铜 SD大鼠 |
Determination of Ca,Mg,Fe,Zn,Cu in SD rat whole blood by flame atomic absorbtion spectrophotoments |
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| WANG Xiaoping , ZHU Qi , JI Xin , DING Yiqing , LI Chunjian , GAO Chao. Determination of Ca,Mg,Fe,Zn,Cu in SD rat whole blood by flame atomic absorbtion spectrophotoments[J]. Journal of Nantong University:Medical sciences, 2012, 32(4): 253-255 |
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| Authors: | WANG Xiaoping ZHU Qi JI Xin DING Yiqing LI Chunjian GAO Chao |
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| Affiliation: | 1(1School of Public Health,2Xinglin College,Nantong University,Nantong 226019) |
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| Abstract: | Objective: Determination of Ca,Mg,Fe,Zn,Cu in sprague dawley(SD) rat whole blood by flame atomic absorbtion spectrophotoments is to built a range of normal value.Methods: The blood sample is treated with concentrated nitric acid and perchloric acid before determination.The method is simple by standard curve method.Results: The results show that recoveries of these microelements are between 90.0% to 110.0%.Conclusion: The result of the method is simple stable and satisfactory. |
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| Keywords: | flame atomic absorbtion spectrophotoments the whole blood Calcium Magnesium Iron Copper Zinc sprague dawley rat |
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