14-3-3蛋白各亚型在大鼠黑质和PC12细胞中的分布

本实验采用免疫组织化学、免疫荧光组织化学和免疫印迹技术对14-3-3蛋白各亚型在大鼠黑质(SN)和PC12细胞中的分布情况进行了研究。在大鼠黑质内,免疫组化和免疫荧光结果显示14-3-3蛋白的γ、ε、θ、ζ四个亚型呈阳性反应,而β和η亚型未见明显阳性反应;在PC12细胞中,6个亚型均呈免疫荧光阳性反应;在黑质中,Westernblot结果显示14-3-3各亚型蛋白表达的相对水平为ε>γ>ζ>θ;而在PC12细胞中各亚型的表达水平为γ>ε>β>ζ>η>θ。本实验的结果提示ε、γ、ζ和θ亚型可能在黑质中发挥主要作用。本研究的结果为研究14-3-3蛋白在Parkinson病中的作用提供了形态学依据。

DISTRIBUTION OF 14-3-3 PROTEIN SUBTYPES IN SUBSTANTIA NIGRA OF RAT AND PC12 CELLS

To investigate the distribution and expression levels of subtypes of 14-3-3 in the substantia nigra (SN) of the rat and PC12 cell, the immunohistochemical, immunofluorescence and immunoblotting methods were used. The results showed that the γ,ε,θ,ζ subtypes of 14-3-3 protein were immunopositively stained, but the evident expression of β and η subtypes were not seen in the SN; while in PC12 cells all the six subtypes (β,η,γ,ε,θ,ζ) were immunopositively stained. Western blot revealed that the[KG-*8] expression[KG-*8] levels[KG-*8] of 14-3-3 protein subtypes in the SN were ε>γ>ζ>θ, while in the PC12 cells the order was γ>ε>β>ζ>η>θ. These results suggest that ε, γ, ζ, and θ subtypes of the 14-3-3 protein might play critical roles in the SN. The results also provide important morphological bases for investigating the role of 14-3-3 protein in Parkinson’s disease.