| 催眠睡茄植株再生体系的建立 |
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| 引用本文： | 罗正伟,孙一铭,吕翠萍,王凤英,孙际薇,孙敏.催眠睡茄植株再生体系的建立[J].中国中药杂志,2012,37(7):897-900. |
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| 作者姓名： | 罗正伟 孙一铭 吕翠萍 王凤英 孙际薇 孙敏 |
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| 作者单位： | 西南大学 生命科学学院 三峡库区生态环境教育部重点实验室,重庆,400715 |
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| 基金项目： | 三峡库区生态环境与生物资源省部共建国家重点实验室培育基地开放基金项目(SKL-2011-04) |
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| 摘 要： | 目的:建立和优化催眠睡茄植株再生体系。方法:以催眠睡茄的叶片作为外植体,探讨不同植物生长物质对愈伤组织诱导,丛生芽分化以及试管苗生根的影响。结果和结论:愈伤组织诱导的最佳培养基是MS+1.0 mg.L-12,4-D+0.1mg.L-1KT;丛生芽诱导的最优培养基是MS+1.0 mg.L-16-BA+0.1 mg.L-1NAA,试管苗生根诱导最优培养基为1/2MS+0.5 mg.L-1NAA;在珍珠岩-腐殖土(1∶1)的基质中,再生植株室外移栽成活率达到92%。
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| 关 键 词： | 催眠睡茄 组织培养 植株再生 |
| 收稿时间： | 2011/9/29 0:00:00 |
Plant regeneration of Withania somnifera |
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| LUO Zhengwei,SUN Yiming,LV Cuiping,WANG Fengying,SUN Jiwei and SUN Min.Plant regeneration of Withania somnifera[J].China Journal of Chinese Materia Medica,2012,37(7):897-900. |
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| Authors: | LUO Zhengwei SUN Yiming LV Cuiping WANG Fengying SUN Jiwei and SUN Min |
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| Institution: | SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China;SchooL of Life Sciense, Southwest University/Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing 400715, China |
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| Abstract: | Objective: To study tissue culture and plant regeneration of Withania somnifera. Method: Leaves of W. somnifera were used for explants,effects of different plant growth substances on callus and shoot induction were studied,different medium and plant growth substances for rooting induction was optimized. Result and Conclusion: The best plant growth substances combination for callus induction was MS+1.0 mg·L-1 2,4-D +0.1 mg·L-1 KT. The optimal medium for germination was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 NAA. The best medium and plant growth substances combination for rooting induction was 1/2MS+0.5 mg·L-1 NAA,transplant survival rate of plantlet reached 92% in humus soil-pearlite(1:1). |
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| Keywords: | Withania somnifera tissue culture plant regeneration |
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