不同提取溶剂对当归赤芍药对主要活性成分溶出率的影响

目的:研究当归赤芍药对不同提取溶媒中活性成分的溶出率。方法:采用UPLC-Q-TOF-MS及UPLC-TQ-MS液质联用集成技术,研究当归赤芍药对中阿魏酸、香草酸、没食子酸等酚酸类成分,芍药苷、芍药内酯苷、羟基芍药苷、没食子酰芍药苷等单萜苷类成分,洋川芎内酯、藁本内酯等苯酞类成分在水及不同浓度乙醇中的溶出率。结果:当归赤芍药对中酚酸类成分,单萜苷类成分随醇浓度的升高溶出率下降,苯酞类成分则反之;在水及低浓度醇中多数活性成分溶出率较高。结论:液质联用集成技术是一种适合在缺少对照品条件下开展多成分综合评价中药制备工艺的可行方法,可为优选工艺参数提供参考。

Influence of different solutions on extracting of active components in Danggui Chishao drug pair

Objective: To study the dissolution rate of active components of different extracting solvents of Danggui Chishao drug pair. Method: The dissolution rates of phenolic acids (ferulic acid, vanillic acid and gallic acid), monoterpenes (gallic acid, peoniflorin, albiflorin, hydroxypeoniflorin and galloylpaeoniflorin) and phthalates (senkyunolide and ligustilide) contained in Danggui Chishao drug pair were determined by quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and ultra high performance liquid chromatography in combination with triple-quadrupole mass spectrometry (UPLC-TQ-MS). Result: The dissolution rates of phenolic acids and monoterpenes decreased with the increase in alcohol concentration, on the contrary the rates of phthalates increased. The relative dissolution rates of most active components were relatively high in water and low concentration alcohol than other solvents. Conclusion: Liquid chromatography-mass spectrometry is practical for comprehensive multi-component assessment on traditional Chinese medicine preparation processes and can provide reference for optimization of processing parameters.