RSLC快速测定多穗柯甜茶中5种甜味成分

多穗柯甜茶中含有多种二氢查尔酮类甜味成分,也是其主要活性成分,为了全面评价多穗柯甜茶的质量,该文选择根皮苷、三叶苷、3’-O-乙酰基根皮苷、2’-O-乙酰基根皮苷、根皮素5种甜味成分为指标进行测定。采用超快速液相色谱法(RSLC),Waters Acquity UPLCBEH C18色谱柱(2.1 mm×100 mm,1.7μm),乙腈-水(25∶75)等度洗脱,流速0.5 mL.min-1,检测波长285 nm,柱温40℃。结果表明5种甜味成分的线性关系良好(>0.9991),线性范围(g.L-1)和加样回收率分别为0.022 2～0.444(98.37%),0.102 8～4.112(97.32%),0.003 39～0.067 68(96.77%),0.005 1～0.204(98.85%)和0.000 538～0.010 76(100.91%)。多穗柯甜茶中这5种成分的质量分数范围(mg.g-1)分别为7.83～62.37,114.24～272.35,0～1.02,0～5.11,0.10～1.19,并且它们的含量变化与样品规格、采收时间呈一定的规律性。该实验方法的灵敏度和准确度均较好,采用等度洗脱能够在6 min内完成1份样品的快速测定,适合多穗柯甜茶中5种成分的快速分析。

Quick determination of five sweet constituents in Duosuike Tiancha by RSLC

Duosuike Tiancha contain multiple dihydrochalcone sweet constituents, which are mainly active constituents. For the purpose of overall assessment on quality Duosuike Tiancha, 5 sweet dihydrochalcones in Duosuike Tiancha, phloridzin, phloretin-4'-beta-D-glucopyranoside, 3'-O-acetylphloridzin, 2'-O-acetylphloridzin and phloretin are determined as indicators. The separation was carried out through a isocratic elution using a Waters Acquity UPLC BRH C18 (2.1 mm x 100 mm, 1.7 microm) column and a mobile phase consisting of water (75%) and acetonitrile (25%) at a flow rate of 0.5 mL x min(-1). The detection wavelength was set at 285 nm. The column temperature was 40 degrees C. Under the optimized conditions, all the 5 sweet constituents were successfully separated with in 6 min, and good linearity (r2 > 0.999 1) was achieved. The linear range (g x L(-)) and recoveries were tested with results of 0.022 2-0.444 (98.37%), 0.102 84. 112 (97.32%), 0.003 39-0.067 68 (96.77%), 0.005 1-0.204 (98.85%) and 0.000 538-0.010 76 (100.91%) respectively. The results indicate that the content of the 5 dihydrochalcones were 7.83-62.37, 114.24-272.35, 0-1.02, 0-5.11 and 0.10-1.19 mg x g(-1), respectively. Furthermore, with certain regularity between their content and the sample size, harvest time. The separation and analysis method are fast and simple, as evidenced by the fact that the gradient elution is adopted to rapidly determine one sample within six minutes. Therefore, it can be used for dsetermine 5 sweet dihydrochalcones Duosuike Tiancha.