腹膜透析腹膜炎动物模型不同时相腹膜溶质转运及IL-8变化

目的 :研究腹膜透析腹膜炎动物模型不同时相腹膜溶质转运及IL 8变化 ,了解腹膜透析溶质转运机制。方法 :将 4 2只新西兰大白兔随机分为实验组 (5min ,2 4h和 4 8h组 )和对照组 ,建立腹膜透析腹膜炎模型。分别测定各时间点透析液和血肌酐、葡萄糖、总蛋白、白蛋白 ,同时测定透析液白细胞介素 8的浓度 ,分别计算肌酐、总蛋白、白蛋白D P值和葡萄糖Dn D0 值。结果 :2 4h组 12 0min ,2 4 0min ,36 0min时白蛋白D P值与对照组比较差异有显著性(P <0 .0 5 ) ;4 8h组各时间点透析液内总蛋白D P值与对照组比较差异有显著性 (P <0 .0 5 ) ;4 8h组各时间点葡萄糖Dn D0 值和肌酐D P值与对照组比较差异有显著性 (P <0 .0 1)。实验组各时间点透析液内白细胞数、中性粒细胞数及IL 8水平升高 ,与对照组比较差异有显著性 (P <0 .0 5 )。 4 8h组各时间点透析液内IL 8水平与总蛋白D P值明显相关 (P <0 .0 5 ) ,6 0min以后各时间点透析液内IL 8水平与白蛋白D P值显著相关 (P <0 .0 1)。结论 :腹膜炎不同时相溶质转运不同 ,IL 8和白细胞均可影响腹膜透析时溶质转运。

Transperitoneal transport of solute and IL-8 in dialysis-related peritonitis

Objective To study the changes in solute transperitoneal transport and the IL 8 level at different time phases in dialysis related peritonitis so as to understand the mechanism of solute transperitoneal transport. Methods Forty two New Zealand white rabbits were randomly divided into three experimental groups (5 min, 24 h, and 48 h groups) and a control group. The rabbit model of peritoneal dialysis related peritonitis was created. The plasma and effluent concentration of creatinine, glucose, total proteins, and albumin were determined respectively; the D/P values of creatinine, total proteins, and albumin, and the Dn/D 0 values of glucose were calculated respectively. Results There was a significant difference in the D/P values of albumin at 120, 240, and 360 min between the 48 h group and the control group P<0.05); the D/P values of total proteins significantly increased at different time points in the 48 h group compared with the control group (P<0.05); there was a significant difference in the creatinine D/P values and glucose Dn/D 0 values between the 48 h group and the control group (P<0.05). Compared with the control group, IL 8 and WBC obviously increased in the 48 h group (P<0.05); the effluent IL 8 levels at different time points were positively correlated to the D/P values of the total proteins (P<0.05). The effluent IL 8 levels at different time points after 60 min were positively correlated to the D/P values of albumin (P<0.05). Conclusion The mechanism of transperitoneal transport of the different molecule solutes varies. Both IL 8 and WBC can influence solute transperitoneal transport in peritoneal dialysis related peritonitis.