非小细胞肺癌组织中Survivin、p16基因的表达及关系

目的 探讨凋亡抑制因子Survivin、抑癌基因p16在非小细胞肺癌(NSCLC)中的表达意义及关系.方法 采用免疫组化链酶亲和素-生物素-过氧化物酶复合体(SABC)方法检测64例NSCLC组织及26例癌旁组织、15例肺良性疾病组织Survivin、p16基因的表达,并分析其相关性.结果 Survivin蛋白在NSCLC组织中阳性表达率70.31%,癌旁组织阳性表达率11.54%(P＜0.05),肺良性疾病中不表达.Survivin蛋白表达与NSCLC的分化程度、TNM分期、淋巴结转移相关(P＜0.05).p16蛋白在肺良性疾病组织及癌旁组织中的表达与在NSCLC组织中的表达差异均有显著性(P＜0.05).p16蛋白表达阳性组、阴性组的Survivin蛋白表达阳性率差异有显著性(P＜0.05),且表达呈正相关(r=0.396,P＜0.005).结论 Survivin基因在NSCLC的发生、发展中起重要作用,有可能作为其早期诊断、治疗的靶基因之一.Survivin的过度表达与p16抑癌作用的失去可能在NSCLC的发病机制中有协同作用.

Expression and relationship of survivin gene and p16 protein in non-small-cell lung cancer

Objective To study the expression and relationship of survivin gene, a novel member of inhibitor of apoptosis protein family, and p16 protein in non-small-cell lung cancer (NSCLC). Methods By strep Avidin-Biotin Complex (SABC) method, the expression of survivin gene and p16 protein was examined in 64 NSCLC tissue samples, 26 paraneoplasmatic tissues and 15 tissue samples of lung benign diseases, and their dependablity was analyzed. Results Survivin expressed in 45 of 65 NSCLC tissues (70.31) (P<0.05) and in 3 of 26 paraneoplasmatic samples (11.54 ) (P<0.05), did not express in the tissue samples of lung benign diseases (P<0.05). Overexpression of survivin was related to differentiation, TNM stages and lymph node metastases of NSCLC (P<0.05). The positive expression of p16 protein was more in paraneoplamatic tissues (76.59), tissues from lung benign diseases (93.33) than in NSCLC tissues (51.56) (P<0.05). The survivin expression in NSCLC was of positive correlation with p16 expression (r=0.396, P=0.005). Conclusion Survivin gene is important in the development and progress of NSCLC. It may become a target gene of early diagnosis and gene therapy for malignant tumor. The overexpression of survivin gene and the deactivation of antioncogene p16 may play synergetic roles in the carcinogenesis of NSCLC.