| 血清登革热病毒NEST-PCR检测 |
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| 引用本文： | 蒋力云,吴新伟,何丽娟,鲁恩洁,刘远,熊远,狄飚.血清登革热病毒NEST-PCR检测[J].预防医学情报杂志,2006,22(3):265-266. |
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| 作者姓名： | 蒋力云 吴新伟 何丽娟 鲁恩洁 刘远 熊远 狄飚 |
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| 作者单位： | 广州市疾病预防控制中心,广东,广州,510080 |
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| 摘 要： | 目的 建立用NEST—PCR快速检测登革热病毒的方法。方法 通用引物对样本进行RT—PCR扩增，然后用分型引物进行NEST—PCR，根据产物条带的位置分型。结果 标准毒株的扩增结果与预期结果相同，血清登革热病毒在病程2～5d的阳性率高于6～9d的阳性率，分别为6／7和2／6。结论 NEST—PCR方法是一个快速、准确检测登革热的方法，但它更适用于早期临床检测。
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| 关 键 词： | 登革热病毒 |
| 文章编号： | 1006-4028(2006)03-265-02 |
| 收稿时间： | 2006-04-13 |
| 修稿时间： | 2006年4月13日 |
Detection of Dengue Virus From Patients's Serums by Nest-PCR |
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| Jiang Li-ytm, Wu Xin-wei, He Li-juan,et al..Detection of Dengue Virus From Patients''''s Serums by Nest-PCR[J].Journal of Preventive Medicine Information,2006,22(3):265-266. |
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| Authors: | Jiang Li-ytm Wu Xin-wei He Li-juan |
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| Institution: | Center for Disease Control and Prevention of Guangzhou city, Guangzhou 510080, China |
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| Abstract: | Objective To set up a rapid method for detection of dengue virus by NEST-PCR. Methods A set of random primers were used for RT-PCT, and then type specified primers were used for NEST-PCR. Types could be identified by the length of the product. Results The amplification result of standard strain were as same as the anticipated result. The positive rate of the early serums (85.7%) was higher than that of the later ones (33.33%). Conclusions The method is a rapid and exact way to detect dengue virus. And it seems more suitable for early clinical detection. |
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| Keywords: | NEST-PCR RT-PCR |
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