重庆市部分高血压患者一氧化氮合酶基因G894T多态性与相关危险因素的研究

目的 了解重庆市部分高血压病患者内皮一氧化氮合酶(eNOS)基因G894T多态性和高血压相关危险因素的关系。方法 高血压患者226例,设对照组。取血标本提取DNA,用聚合酶链反应(PCR)方法扩增目的基因,用限制性内切酶(Ban Ⅱ)酶切PCR产物用于基因分型,同时按照流行病学方法制作规范的调查表,调查内容包括体重指数、膳食、精神心理、疾病家族史等方面情况。结果 eNOS各基因型(GG、GT或TT)在高血压组与对照组中的分布差异无显著性(P>0.05),但在高血压相关的危险因素中(钠盐摄入、性格、打鼾等)分布有差别,T等位基因携带者高血压相关危险因素较多。logistic回归分析显示894T等位基因与高血压发病无关联。结论 eNOS基因G894T多态性在重庆市高血压发病中不起直接重要作用,但可能通过影响相关危险因素参与高血压发病机制。

Study on the relationship between nitric oxide synthase gene G894T polymorphism and hypertension related risk factors in patients with essential hypertension in Chongqing city

OBJECTIVE: To explore the relationship between genetic anomaly and risk factors in hypertension, a polymorphism at position G894T of the gene encoding the endothelial nitric oxide synthase (eNOS) together with hypertension related risk factors were observed in patients with essential hypertension (EH) in Chongqing city. METHODS: Two hundred and twenty-six patients with EH and matched controls were selected. Genotypes of polymorphisms were determined by polymerase chain reaction (PCR), while PCR products were digested by restriction endonuclease (BanII). Questionnaire referred to life style, dietary, smoking, alcohol consumption, psychological and mental state, waist-to-hip ratio (WHR), etc was administered. RESULTS: There was no significant difference noticed in genotype distribution for the eNOS gene G894T genotype between hypertensive groups and controls, but difference was found among certain related risk factors, such as salt intake, snoring and WHR, etc. Logistic regression analysis showed no association between 894T allele and hypertension. CONCLUSION: Although the polymorphism of eNOS gene G894T did not seem to play an important and direct role in the pathogenesis of EH it might have indirect effects through certain risk factors.