Increase in the Level of P-Glycoprotein mRNA Expression in Multidrug-resistant K562 Cell Lines Treated with Sodium Butyrate Is Not Accompanied with Erythroid Differentiation |
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| Authors: | Hiroyuki Shibata Ryunosuke Kanamaru Toshiaki Sato Chikashi Ishioka Yukari Konishi Akira Ishikawa Akira Wakui Takashi Tsuruo |
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| Affiliation: | Department of Clinical Cancer Chemotherapy, The Research Institute for Tuberculosis and Cancer, Tohoku University, 4–1 Seiryomachi, Aoba-ku, Sendai 980;Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113 |
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| Abstract: | We examined the effects of hemin, sodium butyrate and mitomycin C on levels of P-glycoprotein mRNA in human myelogenous K562 cells by northern blot analysis. After treatment with sodium butyrate a dose-dependent increase of P-glycoprotein mRNA expression was observed in the adriamycin-resistant K562 and vincristine-resistant K562 lines. With 10 m M sodium butyrate, the level of P-glycoprotein mRNA reached 20 times that of control adriamycin-resistant K562 and with 30 m M sodium butyrate, it exceeded 5 times that of control vincristine-resistant K562. In contrast, hemin and mitomycin C had almost no effect on P-glycoprotein mRNA. In this experiment, since expression of P-glycoprotein mRNA was not necessarily accompanied with induction of erythroid differentiation, the increased amount of P-glycoprotein mRNA is unlikely to be a result of differentiation. |
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| Keywords: | P-glycoprotein mRNA Multidrug-resistant K562 cell line Sodium butyrate |
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