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抗炎口服液中绿原酸含量的HPLC测定
引用本文:王宏贤. 抗炎口服液中绿原酸含量的HPLC测定[J]. 世界中西医结合杂志, 2007, 2(3): 156-157
作者姓名:王宏贤
作者单位:河南中医学院第一附属医院,河南,郑州,450008
摘    要:目的:建立抗炎口服液质量控制标准。方法:运用ODS色谱柱,以乙腈一0.4%磷酸溶液(13∶87)为流动相,流速1mL/min,检测波长327nm,对抗炎口服液多批样品中的绿原酸进行含量测定。结果:该方法所测的绿原酸含量重现性RSD为1.89%,稳定性高,样品和对照品RSD分别为1.04%和1.28%。结论:该方法准确、稳定、重现性好,可作为抗炎口服液质量控制标准。

关 键 词:高效液相色谱法  抗炎口服液  绿原酸  含量测定
文章编号:1673-6613(2007)03-0156-02
修稿时间:2007-01-08

HPLC Assaying of Chlorogenic Acid in Kang Yan Oral Liquid
Wang Hongxian. HPLC Assaying of Chlorogenic Acid in Kang Yan Oral Liquid[J]. World Journal Of Integrated Traditional and Wesrern Medicine, 2007, 2(3): 156-157
Authors:Wang Hongxian
Affiliation:Wang Hongxian (The 1st Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou, Henan 450008,China)
Abstract:Objective, To establish the quality control standard for Kang Yan Oral Liquid. Methods: The con tent of Chlorogenic Acid in multiple sample of the oral liquid was assayed with High efficiency liquid chromatography (HPLC) by using ODS chromato bar, and under the moving phase with acetonitrile -0.4 phoshoric acid solution (13 : 87), 327 nm of wavelength and 1 mL/min of rate of flow. Results: The reproducibility of the content of chlorogenic acid was 1.8%, the stability in the sample and the control article RSD were respectively 1.04% and 1.28%. Conclusion: This method is accurate, stable and with good reproducibility, and can be used as the quality control standard for Kangyan oral liquid.
Keywords:High efficiency liquid chromatography  Kang yan oral liquid  Cholorogenic acid  Assaying
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