电针对急性心肌缺血的微血管酶和儿茶酚胺荧光的作用 |
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引用本文: | 文琛,曹庆淑.电针对急性心肌缺血的微血管酶和儿茶酚胺荧光的作用[J].针刺研究,1993,18(3):223-227. |
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作者姓名: | 文琛 曹庆淑 |
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作者单位: | 中国中医研究院针灸研究所,中国中医研究院针灸研究所,中国中医研究院针灸研究所,中国中医研究院针灸研究所,中国中医研究院针灸研究所,中国中医研究院针灸研究所,中国中医研究院针灸研究所 北京 100700,北京 100700,北京 100700,北京 100700,北京 100700,北京 100700,北京 100700 |
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摘 要: | 以碱性磷酸酶(ALP)、镁激活的腺苷三磷酸酶(Mg~(2+)-ALP_(ase))和儿茶酚胺(CA)荧光组化方法观察了电针对急性心肌缺血作用的机理。结扎左冠状动脉心室支30分钟后,缺血心肌中以 ATP 显示的毛细血管大为减少,长度缩短;而缺血电针组则有显著意义增加,说明电针对缺血心肌微循环和转运功能的改善。Mg~(2+)-ATP_(ase)可显示心肌微血管的质膜和基膜;经显微分光光度计测量,Mg~(2+)-ATP_(ase)在缺血电针组比缺血对照组光密度增加,这可加强缺血心肌 Na~+K~+离子跨膜转动功能。心肌儿茶酚胺荧光所显示的交感肾上腺素能膨体神经末梢;缺血后减少或耗竭,电针组则较多而亮,这有益于加强缺血心肌的收缩力。
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关 键 词: | 碱性磷酸酶 三磷酸腺苷酶 儿茶酚胺荧光 缺血心肌 |
Effect of EA on the Enzymes of Microvasculature and CA Fluorescence in Acute Myocardial Ischemia |
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Abstract: | It was aimed at observing the histochemical basis of physiological experiment about the effectof electroacupuncture(EA)on acute myocardial ischemia(AMI)in this paper.The capillaries ofmyocardium were reflected with both alkaline phosphatase(ALP)and Mg~(2+)-ATP_(asc).Thirty minafter ligating the left ventricular branch(LVB),the capillaries stained by ALP were much de-creased in the ischemic group without EA,while in EA group they were increased distinctly.Thenumber and the total length of the capillaries in a 220×320μm ischemic area of the posterior wall(section 10μm)were measured and compared.In ischemic group the number was 17.2±1.65,the length was 634.62±666.24μm,in EA group 22.5+1.44,1187.57+103.69μm,respectively.Both counts were significant differences(P<0.05,P<0.001).It reflected that EA could improvethe microcirculation and metabolic function of AMI.Mg~(2+)-ATPase was shown on the plasmmembrance of endothelial cell of microvasculature and analysed quantitatively with a Univar scanning microspectrophotometer.After occluding LVB for 30 min its optic density of AMI was de-creased to 106.834-14.06,while it was increased to 210.83+24.88 in EA group,P<0.05.Theactivity of Mg~(2+)-ATPase could increase in transporting Na(?),k(?)ions through the membrance ofthe endothelial cells of ischemic myocardium.The result was consistent with the physiolgical ex-periment,because EA could regulate the changes of mean repolarization rate induced by AMI andadjust the ion concentration of transmembrane.The catecholamine(CA)in myocardium was lo-cated at sympathetic adrenergic terminals with varicosities containing norepinephrine(NE).Thirty rain after ligating LVB,the fluorescence of adrenergic terminals was decreased and/or de-pleted,but in EA group,the fluorescent varicosities were increased gradually.It was benficial tostrengthing the contractibility of myocardium. |
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