缺血再灌注大鼠海马神经元超微结构的变化

目的:观察缺血再灌注大鼠海马神经元超微结构的改变.方法:雄性SD大鼠32只,随机分为缺血再灌注组和假手术组.缺血再灌注组制备缺血再灌注模型,电镜下观察两组大鼠海马神经元超微结构的变化.结果:缺血再灌注可使海马神经元超微结构尤其是线粒体出现明显损伤;缺血再灌注2h出现的神经元水肿、线粒体肿胀等损伤是可逆的;再灌注6h受损神经元增多,细胞器减少,线粒体外膜、线粒体嵴断裂;再灌注12h神经元损伤更为严重,残存的线粒体崩解呈空泡状.结论:缺血再灌注可损伤大鼠海马神经元超微结构,其作用机制可能与线粒体内Ca2 超载、NOS增加等因素有关.

STUDY ON THE ULTRASTRUCTURE CHANGES OF HIPPOCAMPUS NEURONS IN ISCHEMIA REPERFUSION RATS

Objective:To observe the ultrastructure changes of hippocampus neurons in ischemia reperfusion rats.Methods:32 SD rats were divided into ischemia-reperfusion(IR) group and sham-operation group at random.The IR rat model was made in IR group.The ultrastructure changes of hippocampus neurons were observed under electronmicroscope.Results: IR could make obvious damages on hippocampus neurons,especially for mitochondria.The changes of neurons edema and mitochondria swelling in IR 2h group were reversible.In IR 6h group,the damaged neurons increased: the organelle decreased,the adventitia of mitochondria and the crista mitochondriales broke.In IR 12h group,the damages of neurons were more serious: the remaining mitochondria were in vacuole-shape. Conclusions: IR can damage the ultrastructure of hippocampus neurons,the mechanisms may has relationship with overload of Ca~(2+) and increasing of NOS in mitochondria.