ATP敏感性钾通道在乳化异氟醚心肌保护效应中的作用

目的探讨ATP敏感性钾通道(KATP)是否参与乳化异氟醚(EI)对心肌细胞的保护作用。方法原代培养乳鼠离体心肌细胞,建立缺氧/复氧(H/R)损伤模型,随机分为6组,正常组(N组)、缺氧/复氧组(H/R组)、H/R+脂肪乳组(F组)、H/R+10mmol.L-1格列本脲组(G组)、H/R+1.68mmol.L-1EI组(EI组)和H/R+1.68mmol.L-1EI+10mmol.L-1格列本脲组(EIG组),n=6。各组取细胞培养上清液测定LDH活力与cTnI含量,心肌细胞匀浆后测定SOD活力与MDA含量;用倒置显微镜观察心肌细胞生长特性及形态的变化。结果与N组比较,各组的LDH、MDA、cTnI均升高(P<0.05),SOD下降(P<0.05)。与H/R组比较,F组的SOD下降(P<0.05),LDH、MDA、cTnI的差异均无统计学意义(P>0.05),G组的SOD、LDH、MDA、cTnI的差异均无统计学意义;EI组和EIG组的LDH、MDA、cTnI均降低(P<0.05),SOD升高(P<0.05)。与F组比较,EI组和EIG组的LDH、MDA、cTnI均下降(P<0.05),SOD升高(P<0.05)。与EI组比较,EIG组的LDH活力、MDA和cT-nI含量均升高,SOD活力降低(P<0.05)。结论乳化异氟醚对原代培养心肌细胞缺氧/复氧损伤的保护作用可能与其激活ATP敏感性钾通道有关。

Contribution of ATP-sensitive K+ channel to emulsified isoflurane's protective effect on myocardial cells

Aim To discuss whether the ATP-sensitive K+ channel participates in the protective effect of the emulsified isoflurane(EI) on myocardial cells.Method The in-vitro neonate rat cardiac myocytes were primarily cultured,the H/R injury models were established and randomly divided into 6 groups,namely,normal control group(N group),H/R group(H/R group),H/R+fat emulsion group(F group),H/R+glibenclamide group(G group),H/R+1.68 mmol·L-1 EI group(EI group),and H/R+1.68 mmol·L-1 EI +10 mmol·L-1+glibenclamide group(EIG group).Supernatant liquid of cell culture was taken from each group(n=6) to mensurate LDH and cTnI,and myocardial cell homogenate was used to mensurate SOD and MDA;the changes in the characteristics and forms of myocardial cell growth were observed with inverted microscope.Results Compared to N group,the LDH,MDA and cTnI of other groups all increased(P<0.05) and SOD declined(P<0.05).Compared to H/R group,SOD of F group declined(P<0.05),and the differences of the LDH,MDA and cTnI had no statistic significance(P>0.05);the differences of G group's LDH,MDA,SOD and cTnI had no statistic significance(P>0.05);LDH,MDA and cTnI of EI and EIG group declined(P<0.05) and SOD increased(P<0.05).Compared to F group,the LDH,MDA and cTnI of EI and EIG group declined(P<0.05) and SOD increased(P<0.05).Compared to EI group,the LDH,MDA and cTnI of EIG group increased(P<0.05) and SOD declined(P<0.05).Conclusion The protective effect of emulsified isoflurane(EI) on the primarily cultured myocardial cells hypoxia/reoxyenation injury may be associated with the ATP-sensitive K+ channel activated by it.