p53、Cyclin D1及PCNA在非小细胞肺癌的表达

目的 研究非小细胞肺癌（NSCLC）中p53、Cyclin D1蛋白表达及与细胞增殖的关系。方法 应用免疫组织化学技术（SP法）检测74例NSCLC中p53、Cyclin D1蛋白和增殖细胞抗原（PCNA）的表达情况。结果 p53蛋白、CyclinD1蛋白在NSCLC中阳性表达率分别为55．41％和37．84％,均明显高于正常肺组织（P1＝0．0031,P2＝0．0429）。 p53蛋白表达与淋巴结转移有密切关系（P＝0．0222）。p53蛋白、CyclinD1蛋白表达分别与PCNA指数有密切关系（P1＝0．001,P2＝0．0009）。p53蛋白与CyclinD1蛋白表达之间未见明显关系（P＞0．05）。结论 p53蛋白和CyclinD1蛋白近表达参与了NSCLC的发生发展,有促进细胞增殖的作用。p53的诊断和评价NSCLC预后的重要参数。

The expression of p53, cyclin D1 and PCNA in non-small cell lung cancer

Objective To study the expression of p53, cyclin D1 proteins and their relations to cell proliferation in non small cell lung cancer (NSCLC). Methods The expression of p53, cyclin D1 proteins and PCNA was studied in 74 cases of NSCLC and 10 cases of normal lung tissues by SP immunohistochemical technique. Results Positive rates of p53 and cyclin D1 protein expression in NSCLC were 55.41% and 37.84% respectively, which were both significantly higher than those in normal lung tissues (P 1=0.003?1, P 2=0.042?9). p53 protein expression correlated remarkably with lymphatic metastasis (P=0.022?2). p53 and cyclin D1 protein expression correlated significantly with PCNA index respectively (P 1=0.001?0,P 2=0.000?9). There was no significant correlation between p53 and cyclin D1 proteins (P> 0.05) . Conclusion p53, cyclin D1 protein overexpression plays an important role in tumorigenesis and cell proliferation of NSCLC. p53 is also an important indicator in the diagnosis and prognostic evaluation of NSCLC.