| 芦荟多糖的分离提取与含量测定 |
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| 引用本文： | 林新华,陈俊,陈伟,黄丽英,罗红斌.芦荟多糖的分离提取与含量测定[J].福建医科大学学报,2003,37(2):214-216. |
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| 作者姓名： | 林新华 陈俊 陈伟 黄丽英 罗红斌 |
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| 作者单位： | 福建医科大学药学系,福州,350004 |
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| 基金项目： | 福建省科技厅国际合作项目 ( 2 0 0 2 I0 15 ) |
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| 摘 要： | 目的 从芦荟中分离提取芦荟多糖并对其含量进行测定。方法 采用先醇沉后水提的方法分离提取芦荟多糖，以甘露糖标准液绘制标准曲线，用苯酚-硫酸分光光度法测定多糖含量。结果 芦荟各部位多糖含量不同，凝胶、叶皮和全叶的多糖含量分别为0．997，1．66，1．08mg／g。结论 该测定方法准确、重复性好，对芦荟多糖含量的测定获得满意结果。
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| 关 键 词： | 芦荟 多糖类 甘露糖 分光光度法 植物提取物 |
| 文章编号： | 1672-4194(2003)02-0214-03 |
| 修稿时间： | 2003年1月29日 |
Extraction and Determination of Polysaccharide from Aloe |
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| LIN Xin\|hua,CHEN Jun,CHEN Wei,HUANG Li\|ying,LUO Hong\|bin Division of Pharmacy,Fujian Medical University,Fuzhou ,China.Extraction and Determination of Polysaccharide from Aloe[J].Journal of Fujian Medical University,2003,37(2):214-216. |
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| Authors: | LIN Xin\|hua CHEN Jun CHEN Wei HUANG Li\|ying LUO Hong\|bin Division of Pharmacy Fujian Medical University Fuzhou China |
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| Institution: | LIN Xin\|hua,CHEN Jun,CHEN Wei,HUANG Li\|ying,LUO Hong\|bin Division of Pharmacy,Fujian Medical University,Fuzhou 350004,China |
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| Abstract: | Objective To extract polysaccharide from Aloe. Methods The polysaccharide was purified by precipitation with ethanol followed by extraction with distilled water. Its total soluble sugar content was detected colorimetrically by the phenol\|sulfuric acid method with mannose as standard. \{Results\} The soluble sugar contents of gel, skin and whole leave were 0 997, 1 66 and 1 08 mg/g, \{respectively\}. Conclusion This method is accurate and well reproducible. |
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| Keywords: | Aloe polysaccharides mannose spectrophotometry plant extract |
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