莱菔硫烷抑制氧化应激下人视网膜色素上皮细胞凋亡

目的：观察仅莱菔硫烷（SFN）对外源性H2O2诱导的人视网膜色素上皮细胞ARPE-19细胞凋亡的影响，并探讨其可能的分子机制。方法：体外培养人视网膜色素上皮细胞系ARPE-19细胞，用12．5mmol／L外源性过氧化氢（H2O2）作用30min后，再加入不同浓度SFN作用不同时间。流式细胞术检测ARPE-19细胞凋亡，RT—PCR和Westernblot分别检测H0-1mRNA和蛋白的表达。同时分别采用HO-1的激动剂CoPP和抑制剂ZnPP处理细胞，观察细胞凋亡的变化。结果：SFN处理ARPE-19细胞后，可显著诱导其表达H0—1mRlqA和蛋白，并呈一定的剂量依赖性。同时，H2O2处理后能显著诱导ARPE-19细胞产生ROS，而经不同浓度SFN处理后，ROS产生显著降低。结论：α—硫辛酸诱导氧化应激下人视网膜色素上皮细胞表达H0-1，从而抑制ROS的过度产生。

Apoptosis of retinal pigment epithelial cells under sulforaphane inhibiting oxidation

Objective： To observe the influence of only sulforaphane （SFN） on the exogenous H2O2 inducing the apoptosis of retinal pigment epithelial cells ARPE-19 and explore its possible molecular mechanism. Methods： ARPE-19 cells of retinal pigment epithelial cell were cultivated. With 12.5mmol/L exogenous hydrogen peroxide （H2O2） for 30min, different concentration of SFN was added at different time. ARPE-19 cell apoptosis was detected by flow cytometry and the expression of HO-1 mRNA and protein were detected by RT-PCR and Western blot. At the same time, the changes of cell apoptosis were observed by byagonist CoPP and inhibitor ZnPP of HO-1. Results： After treated ARPE-19, SFN can significantly induced the expression of HO-1 mRNA and protein, which is dependent on dose. Meanwhile, H2O2 treatment could significantly induce ARPE-19 cells to produce ROS, but with different concentration of SFN, ROS significantly decreased. Conclusion： ct-lipoic acid inducing oxidative in retinal pigment epithelial cells expresses HO-1 in order to inhibit the overproduction of ROS.