野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元、染料木素的含量比较

目的：比较野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元和染料木素的含量。方法：样品以30%乙醇回流提取后采用高效液相色谱法测定含量。色谱柱为Uvis-201（250mm×4.6mm,5μm）,流动相为甲醇-水（梯度洗脱）,流速为1.0mL·min-1,检测波长为250nm,柱温为室温。结果：葛根素、大豆苷、大豆苷元、染料木素的进样量分别在0.68～5.44μg（r=0.9992）、0.143～1.144μg（r=0.9993）、0.0245～0.1960μg（r=0.9992）、0.088～0.704μg（r=0.9994）范围内与各自峰面积积分值呈良好线性关系;平均加样回收率分别为98.1%、99.5%、101.1%、99.2%,RSD分别为2.17%、2.42%、2.53%、2.65%（n均为6）。葛根素含量高低排序为河南野葛〉云南葛〉山西野葛〉大别山野葛〉广西粉葛;大豆苷含量为云南葛〉河南野葛〉大别山野葛〉山西野葛〉广西粉葛;大豆苷元含量为河南野葛〉云南葛〉山西野葛〉大别山野葛〉广西粉葛;染料木素含量为河南野葛〉云南葛〉山西野葛〉大别山野葛〉广西粉葛。结论：野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元、染料木素含量差别较大,且云南葛为野葛的伪品之一,三者不能同等应用。

Comparison of Puerarin, Daidzin, Daidzein, Genistein Content of Pueraria lobata,P. thomsonii and P. peduncularis

OBJECTIVE：To compare the content of puerarin, daidzin, daidzein and genistein from Pueraria lobata,P. thomsonii and P. peduncularis. METHODS： After reflux extraction of 30% ethanol, the sample was determined by HPLC. The determination was performed on Uvis-201 （250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-water （gradient elution） at the flow rate of 1.0 mL·min-1. The detection wavelength was 250 nm and column temperature was ambient temperature. RESULTS： The linear range was 0.68～5.44 μg for puerarin（r=0.999 2）,0.143～1.144 μg for daidzin （r=0.999 3）, 0.024 5～0.196 0 μg for daidzein（r=0.999 2） and 0.088～0.704 μg（r=0.999 4） for genistein; average recovery rates were 98.1%,99.5%,101.1% and 99.2%,RSDs were 2.17%,2.42%,2.53% and 2.65%（n=6）. The content of puerarin was in descending order：P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi; the content of daidzin was in descending order：P. peduncularisP. lobata in HenanP. lobata in DabieshanP. lobata in ShanxiP. thomsonii in Guangxi; the content of daidzein was in descending order：P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi; the content of genistein was in descending order： P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi. CONCLUSION： The contents of puerarin, daidzin, daidzein and genistein in P. lobata, P. thomsonii and P. peduncularis were different greatly, and P. peduncularis is a false one of P. lobata. The use of them should be different.