| 用流式细胞仪对胸水细胞的DNA含量和细胞周期的研究 |
| |
| 引用本文： | 沈策,叶曜芩,罗文侗,孔宪涛.用流式细胞仪对胸水细胞的DNA含量和细胞周期的研究[J].中华结核和呼吸杂志,1994(2). |
| |
| 作者姓名： | 沈策 叶曜芩 罗文侗 孔宪涛 |
| |
| 作者单位： | 上海第二军医大学附属长征医院 |
| |
| 摘 要： | 用流式细胞仪（ＦＣＭ）对胸腔积液患合同一次胸水细胞ＤＮＡ含量、染色体和病理细胞学的研究表明：１８例癌性胸水的非整倍体ＤＮＡ含量出现率为８３．３３％，１０例结核性胸水均为二倍体ＤＮＡ含量。ＦＣＭ检测细胞ＤＮＡ含量和细胞周期分析方法的诊断阳性率最高，特异性最好，染色体次之，细胞学最低。反复多次细胞学检查可显著提高阳性率。对病理细胞学可疑的病例，有条件可行ＤＮＡ或染色体检查，有助于确诊。
|
| 关 键 词： | 流动血细胞计数，胸膜积液，脱氧核糖核酸，染色体 |
Relativ DNA content and cycle distribution ofcells in pleural effusions studied by flow-cytometry. |
| |
| Shen Ce, Ye Yao- qin, Lou Wen-tong et al..Relativ DNA content and cycle distribution ofcells in pleural effusions studied by flow-cytometry.[J].Chinese Journal of Tuberculosis and Respiratory Diseases,1994(2). |
| |
| Authors: | Shen Ce Ye Yao- qin Lou Wen-tong |
| |
| Abstract: | leural effusions from 28 patients were studied bycytology,chromosome and flow-cytometry.Relativecellular DNA content was measured. An estimate ofploidy as well as cyclc distribution has been given.Eighteen patients sufferred from malignant effusionswhile the rest had TB effusions. 83.3% of maligananteffusion had aneuploid.The relative eellular DNAcontent and the cell cycle distribution can providevaluable and decisive information. The study ofchromosome also help the diagnosis in addition tocytological results. |
| |
| Keywords: | Flow cytometry Pleural effu-sion DNA Chromosomes |
| 本文献已被 CNKI 等数据库收录！ |
