| 实验性肝纤维化胶原变化的研究 |
| |
| 引用本文： | 陈东风,刘为纹.实验性肝纤维化胶原变化的研究[J].胃肠病学和肝病学杂志,1997,6(4):321-323,331. |
| |
| 作者姓名： | 陈东风 刘为纹 |
| |
| 作者单位： | 第三军医大学附属大坪医院消化内科!重庆400042(陈东风，冷恩仁)，第三军医大学附属西南医院消化科!重庆630038(刘为纹) |
| |
| 摘 要： | 目的：研究肝纤维化过程中胶原成分的变化规律。方法：制作四氯化碳肝纤维化模型，测定肝组织羟脯氨酸含量，用ＶＧ、ＲＦ染色显示Ⅰ、Ⅱ型胶原并进行计算机图像分析，放免法测定肝匀浆及血清Ⅲ型前胶原水平，应用上述指标反映肝纤维化过程中胶原的变化规律。结果：随实验时间延长，肝内胶原含量逐渐增加。肝纤维化早、中期以Ⅲ型胶原增生为主，肝纤维化后期及肝硬化期以Ⅰ期胶原为增生为主。血清ＰＣⅢ与肝内胶原变化基本同步。结论
|
| 关 键 词： | 肝纤维化 胶原 Ⅲ型前胶原 肝硬变 |
Studies of collagen in ccl4-induced rat liver fibrosis |
| |
| Chen Dongfeng,Leng Enren,Liu Weiwen.Studies of collagen in ccl4-induced rat liver fibrosis[J].Chinese Journal of Gastroenterology and Hepatology,1997,6(4):321-323,331. |
| |
| Authors: | Chen Dongfeng Leng Enren Liu Weiwen |
| |
| Institution: | Chen Dongfeng,Leng Enren,Liu Weiwen Department of Gastroenterology,Daping Hospital,Third Military Medical University,Chongqing,400042 |
| |
| Abstract: | Aims:To research the changes of collagen during the period of development of liver fibrosis induced by CCL4 in rats. Methods: The contents of hepatic hydroxyproline, volume density of collagen type I and type III, the levels of procollagen type III (PCIII) in sera and liver homogenate were measured.The histological findings were also observed by light and electron microscopy.Results:The quantity of collagen in hepatic parenchyma increased gradually. In the early and middle stages of fibrosis, the amounts of type III collagen significantly increased in liver, however, in the final fibrotic stage and cirrhotic stage, type I collagen increased dominately. Conclusions: These results indicated that increases of collagen in liver were a basic pathological process in liver fibrosis and the levels of PCIII in sera might be useful parameter to diagnose the liver fibro- |
| |
| Keywords: | Liver fibrosis Collagen Procollagen type III |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
