Intraspecies genotype variability of the microsporidian parasite Encephalitozoon hellem |
| |
Authors: | Haro María Del Aguila Carmen Fenoy Soledad Henriques-Gil Nuno |
| |
Institution: | Departamento de Biología Celular, Bioquímica y Biología Molecular, Facultad de Ciencias Experimentales y de la Salud, Universidad San Pablo-CEU, 28668 Boadilla del Monte, Madrid, Spain. |
| |
Abstract: | Seven isolates of Encephalitozoon hellem from human immunodeficiency virus-positive patients were genotyped through a series of markers: the internal transcribed spacer (ITS) of ribosomal DNA, the polar tube protein (PTP) gene, and two intergenic spacers (IGS-TH and IGS-HZ) whose polymorphism is newly reported. The genome markers were all analyzed at three levels: PCR amplification followed by polyacrylamide gel electrophoresis, single-strand conformation analysis (SSCA), and DNA sequencing. The polymorphisms detected involve insertions/deletions and point mutations. SSCA can distinguish any pair of sequences, even those differing by a single base pair. The different isolates studied fit into the previously described ITS genotype 1A, except one which seems to be a 2A derivative variant (2D). When PTP and the new markers IGS-TH and IGS-HZ were analyzed, most of the isolates displayed different genotypes, demonstrating that E. hellem has a strong intraspecies variability. A set of markers such as those used here may be very useful in genotyping of clinical samples and in the assessment of epidemiological relationships among E. hellem strains. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|