Quantification of Circulating Cell-Free DNA in the Serum of Patients with Obstructive Sleep Apnea–Hypopnea Syndrome |
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Authors: | Liang Ye Guan-Hua Ma Ling Chen Min Li Jia-Lin Liu Kun Yang Qing-Yun Li Ning Li Huan-Ying Wan |
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Institution: | (1) Department of Respiratory Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197, Ruijin Second Road, Shanghai, 200025, China; |
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Abstract: | Serum cell-free DNA concentrations have been reported to increase in many acute diseases as well as in some chronic conditions
such as cancer and autoimmune diseases. The aim of this study was to examine whether serum DNA concentrations were elevated
in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). The effects of nasal continuous positive airway pressure
(nCPAP) on serum DNA were also investigated. One hundred twenty-seven people diagnosed with OSAHS by polysomnography (PSG)
were admitted into the OSAHS group, and 52 subjects without OSAHS were recruited for the control group. The OSAHS group was
further divided into mild, moderate, and severe OSAHS subgroups based on their apnea-hypopnea index (AHI) during sleep. Ten
patients with moderate and severe OSAHS were treated with nCPAP. Serum DNA, interleukin-6 (IL-6), and malonaldehyde (MDA)
concentrations were measured and were found to be significantly higher in patients with moderate and severe OSAHS groups than
those in the mild OSAHS and control groups (p < 0.05). Univariate analysis showed that serum DNA correlated positively with AHI, oxygen desaturation index (ODI), IL-6,
and MDA, and negatively correlated with minimal oxygen saturation (miniSaO2) (all p < 0.05). In stepwise multiple regression analysis, only MDA and miniSaO2 were suggested as significant independent predictors for the serum DNA concentrations. After 6 months of nCPAP therapy, serum
concentrations of DNA, IL-6, and MDA were significantly decreased (p < 0.05). The increasing concentration of serum DNA in patients with OSAHS was positively correlated with disease severity.
Serum DNA may become an important parameter for monitoring the severity of OSAHS and effectiveness of therapy. |
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