胎鼠神经干细胞移植对阿尔茨海默病大鼠海马区的GFAP与S-100β表达的影响

目的探讨胎鼠神经干细胞移植对阿尔茨海默病(AD)大鼠海马区的GFAP与S-100β表达的影响。方法通过切断Wistar大鼠海马穹窿伞制备AD模型大鼠。我们将大鼠随机分为神经干细胞移植组、生理盐水对照组和正常组。培养胎鼠的神经干细胞,将其移植入AD模型大鼠脑内,并用等量生理盐水脑内注射对比观察。1个月后处死大鼠,进行免疫组织化学实验检测鼠海马区的GFAP与S-100β的表达。结果神经干细胞显示巢蛋白阳性。各组实验鼠海马区有GFAP的阳性表达,生理盐水对照组与其他各组之间有显著差异(P<0.01)。对各组大鼠在海马区均可见棕褐色的S-100β阳性细胞存在,部分细胞呈细胞质性染色阳性。生理盐水对照组AD模型大鼠的海马区切片上可见大量阳性细胞染色,与其他各组比较差异有显著性(P<0.01)。结论神经干细胞移植可以促进AD模型大鼠脑内神经元更好的恢复。

Effects of fetal rat neural stem cells (NSCs) transplantation on expression of GFAP and S-100β in hippocampus of rats with Alzheimer''''s disease

Objective To observe the effects of fetal rat NSCs transplantation on expression of GFAP and S-100β in hippocampus of rats with AD.Methods The AD rat model whose hippocompus was damaged by operation was established and NSCs taken from hippocampus of fetal rats were cultured.After the Alzheimer model was established,the rats were randomly divided into NSCs transplantation group,physiological saline control group and normal group.Four weeks after NSCs transplantation,the expression of GFAP and S-100β in hippocampus of rats with AD was examined by immunohistochemical technique.Results Nestin positive proved that the cells were NSCs.After transplanting NSCs into the hippocampus,the amount of GFAP-postive astrocytes increased in hippocampus of the saline control group and was significantly different from that of other groups(P<0.01).S-100β-postive cells existed in hippocompus of all groups.The S-100β-postive cells of the saline control group had significant difference from that of the NSCs transplantation group and normal group(P<0.01).Conclusion NSCs transplantation as a promising cell therapy has effects on the neurons in the lesion area and makes the nerve connections recover better.